Evidence map›Paper›PMID 27044072›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2016

Axial superresolution via multiangle TIRF microscopy with sequential imaging and photobleaching.

Yan Fu, Peter W Winter, Raul Rojas, Victor Wang, Matthew McAuliffe, George H Patterson

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
17.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 62 citations in OpenAlex.

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  8. Spectroscopic Methods Used in Implant Material Studies.Molecules (Basel, Switzerland) · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Yan FuSection on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892;
Peter W WinterSection on High-Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892;
Raul RojasSection on Biological Chemistry, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892;
Victor WangBiomedical Imaging Research Services Section, Division of Computational Bioscience, Center for Information Technology, National Institutes of Health, Bethesda, MD 20892.
Matthew McAuliffeBiomedical Imaging Research Services Section, Division of Computational Bioscience, Center for Information Technology, National Institutes of Health, Bethesda, MD 20892.
George H PattersonSection on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892; pattersg@mail.nih.gov.
National Institutes of Health · USNational Institute of Biomedical Imaging and Bioengineering · USNational Institute of Dental and Craniofacial Research · US

Funding

Scientific Applications Services SectionZIACT000272 · CIT · CENTER FOR INFORMATION TECHNOLOGY · PI MCAULIFFE, MATTHEW · 2009 to 2021
$9.9M
BiophotonicsZIAEB000071 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI PATTERSON, GEORGE · 2010 to 2022
$9.7M
Intramural NIH HHS
6 · The paper itself

Abstract

We report superresolution optical sectioning using a multiangle total internal reflection fluorescence (TIRF) microscope. TIRF images were constructed from several layers within a normal TIRF excitation zone by sequentially imaging and photobleaching the fluorescent molecules. The depth of the evanescent wave at different layers was altered by tuning the excitation light incident angle. The angle was tuned from the highest (the smallest TIRF depth) toward the critical angle (the largest TIRF depth) to preferentially photobleach fluorescence from the lower layers and allow straightforward observation of deeper structures without masking by the brighter signals closer to the coverglass. Reconstruction of the TIRF images enabled 3D imaging of biological samples with 20-nm axial resolution. Two-color imaging of epidermal growth factor (EGF) ligand and clathrin revealed the dynamics of EGF-activated clathrin-mediated endocytosis during internalization. Furthermore, Bayesian analysis of images collected during the photobleaching step of each plane enabled lateral superresolution (<100 nm) within each of the sections.

Indexed as

Imaging, Three-DimensionalPhotobleachingCell LineClathrinEndocytosisEpidermal Growth FactorFluorescent DyesHumansMicroscopy, FluorescenceClathrinEpidermal Growth FactorFluorescent Dyesaxial superresolutionphotobleachingtotal internal reflection fluorescence

Identifiers

PMID27044072
PMCPMC4843470
OpenAlexW2315524369

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.