Evidence map›Paper›PMID 27042699›Full record

ArticleMOJ proteomics & bioinformatics

Proteomics Characterization of Primary Human Oral Epithelial Cells Using a Novel Culture Technique for Use in Tissue Regeneration.

Hiroko Kato, Andy Lo, Shiuhyang Kuo, Song Nie, Cynthia L Marcelo, David M Lubman, Stephen E Feinberg

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MOJ proteomics & bioinformatics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Hiroko KatoDepartment of Oral and Maxillofacial Surgery, University of Michigan, USA; Department of Biomimetics, Niigata University Postgraduate School of Medical and Dental Sciences, Japan.
Andy LoDepartment of Surgery, University of Michigan Medical Center, USA.
Shiuhyang KuoDepartment of Oral and Maxillofacial Surgery, University of Michigan, USA.
Song NieDepartment of Surgery, University of Michigan Medical Center, USA.
Cynthia L MarceloDepartment of Oral and Maxillofacial Surgery, University of Michigan, USA.
David M LubmanDepartment of Surgery, University of Michigan Medical Center, USA.
Stephen E FeinbergDepartment of Oral and Maxillofacial Surgery, University of Michigan, USA.
University of Michigan–Ann Arbor · US

Funding

Microsequencing in an Ion Trap/reTOF DeviceR01GM049500 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LUBMAN, DAVID M. · 1994 to 2016
$3.0M
NIGMS NIH HHS R01 GM049500
6 · The paper itself

Abstract

Oral mucosa keratinocytes are widely used in regenerative medicine. The unique cultured cell population "Epithelial-derived Pop-Up Keratinocytes (ePUKs)" was previously reported as undifferentiated cells. Gravity Assisted Cell Sorting (GACS) was used to isolate a small-sized population of undifferentiated cells enriched ePUKs. LC/MS/MS analysis was performed to define the cellular profile of ePUKs of primary human oral mucosa keratinocytes. Small sized ePUKs which showed increased expression of Dickkopf WNT signaling pathway inhibitor 1 (DKK1), serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 (SERPINE1), follistatin and tenascin-C were verified by Western blots. These proteins are involved in the regulation of cellular movement, hair follicle development and the maintenance of its stem cell niche. The fabrication of a tissue-engineered oral mucosa, ex vivo produced oral mucosa equivalent (EVPOME), using ePUKs showed increased abundance of these verified proteins. These findings indicate that the specific phenotype of ePUKs and their ability to influence wound healing promotion are implicated by highly expressed cellular movement regulatory proteins. Therefore, ePUKs may be a useful cell source for use in regenerative medicine.

Indexed as

Oral mucosaQuantitative proteomicsTissue engineering

Identifiers

PMID27042699
PMCPMC4813815
OpenAlexW2190255357

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.