ArticleJournal of clinical and diagnostic research : JCDR2016
Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha.
Article in Journal of clinical and diagnostic research : JCDR, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Prevalence and characterization of bacterial rectal colonization patterns in pediatric patients: A cross-sectional study.New microbes and new infections · 2026Article
- Antimicrobial resistance profiles of non-aureus Staphylococci isolated from farm animals, farm environments and companion animals.Scientific reports · 2026Article
- Exploring the incidence potential of phenol-soluble modulins among methicillin-resistant Staphylococcus aureus in Lahore, Pakistan.BMC infectious diseases · 2025Article
- Methicillin-resistantCurrent pharmaceutical biotechnology · 2025Review
- Article
- Phenotypic and genotypic characterization of methicillin resistantIranian journal of microbiology · 2024Article
- Effect of silica-sprayed collection tubes on synovial fluid bacterial culture.Veterinary medicine and science · 2024Article
- Prevalence of methicillin-resistantFrontiers in veterinary science · 2022Article
- Comparison of PCR and phenotypic methods for the detection of methicillin resistantIranian journal of microbiology · 2021Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionMethicillin resistant Staphylococcus aureus has emerged as an important pathogen in nosocomial and community acquired infections. Accurate and rapid identification of MRSA in clinical specimens is essential for timely decision of effective antimicrobial chemotherapy.
aimThe present study was conducted to compare efficacy of four conventional phenotypic methods, with mec- A based polymerase chain reaction (PCR) for MRSA identification. MATERIALS AND
methodsMethicillin resistance was determined in 200 S.aureus isolates by oxacillin disc diffusion, cefoxitin disc diffusion, Oxacillin Resistance Screening Agar and E-test. The results were compared with mec-A based PCR.
resultsAmong 200 S.aureus isolates 62 (31%) were positive for mec-A gene by PCR. Cefoxitin disc diffusion, Oxacillin Resistance Screening Agar and E-test showed 100% specificity. Oxacillin disc diffusion had lowest sensitivity (82.5%) and specificity (98.5%) among all. The conventional methods take more time than PCR for diagnosing MRSA. Linezolid, Vancomycin & Dalfopristin were the highly sensitive drugs against MRSA isolates.
conclusionCefoxitin disc diffusion, is rapid, simple and cheaper, hence can be used routinely as an alternative to PCR for detection of MRSA in resource constraint laboratories.
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