ArticleClinical and translational allergy2016
The expression of CD123 can decrease with basophil activation: implications for the gating strategy of the basophil activation test.
Article in Clinical and translational allergy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- ConvexGating infers gating strategies from clusters in single cell cytometry data.Nature communications · 2026Article
- Standardizing a Four-Marker Basophil Activation Test: Influence of Storage Time, Temperature, and IgE Levels.Journal of asthma and allergy · 2026Article
- Basophil Activation Test in IgE-Mediated Wheat Allergy: Diagnostic and Clinical Applications-A Narrative Review.Diagnostics (Basel, Switzerland) · 2025Review
- A hand-operated microfluidic sample preparation-to-analysis workflow for simplifying the basophil activation test.Lab on a chip · 2025Article
- Applications of basophil activation test in paediatric allergic diseases.The World Allergy Organization journal · 2024Review
- Allergenic Biomarkers in the Molecular Diagnosis of IgE-Mediated Wheat Allergy.International journal of molecular sciences · 2024Review
- Combining avidin with CD63 improves basophil activation test accuracy in classifying peanut allergy.Allergy · 2024Article
- Towards standardizing basophil identification by flow cytometry.Frontiers in allergy · 2023Article
- Review
- Increased expressions of CD123, CD63, CD203c, and Fc epsilon receptor I on blood leukocytes of allergic asthma.Frontiers in molecular biosciences · 2022Article
- Role of Chemokines and Inflammatory Cells in Respiratory Allergy.Journal of asthma and allergy · 2022Review
- Basophil Activation Test Reduces Oral Food Challenges to Nuts and Sesame.The journal of allergy and clinical immunology. In practice · 2021Article
- Behind the scenes with basophils: an emerging therapeutic target.Immunotherapy advances · 2021Review
- Biomarkers of severity and threshold of allergic reactions during oral peanut challenges.The Journal of allergy and clinical immunology · 2020Article
- Quantification of Early-Stage Myeloid-Derived Suppressor Cells in Cancer Requires Excluding Basophils.Cancer immunology research · 2020Article
- The Absolute Basophil Count.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Road map for the clinical application of the basophil activation test in food allergy.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2017Review
- Commentary: The Expression of CD123 Can Decrease with Basophil Activation: Implications for the Gating Strategy of the Basophil Activation Test.Frontiers in immunology · 2016Article
- Basophil activation test: food challenge in a test tube or specialist research tool?Clinical and translational allergy · 2016Review
Corrections and comments
- Commented on by
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundBasophil activation test (BAT) reproduces IgE-mediated allergic reactions in vitro and has been used as a diagnostic test. Different markers can be used to identify basophils in whole blood and have implications for the outcome of the test. We aimed to assess changes in the expression of CD123 and HLA-DR following basophil activation and to select the best gating strategy for BAT using these markers.
methodsBAT was performed in whole blood from 116 children. Peanut extract, anti-IgE, anti-FcεRI or formyl-methionyl-leucyl-phenylalanin (fMLP) was used for stimulation. Surface expression of CD123, HLA-DR, CD63 and CD203c was evaluated by flow cytometry.
resultsIn some cases, gating on CD123+/HLA-DR- led to the loss-to-analysis of basophils in conditions where basophils were activated. Adding CD203c as an identification marker restored the cell number. Basophils remained HLA-DR-negative with activation. CD123 expression decreased following stimulation with fMLP (n = 116, p < 0.001), anti-IgE (n = 104, p < 0.001) and peanut (n = 42, p < 0.001). The decrease in the mean fluorescence intensity of CD123 correlated with the up-regulation of basophil activation markers, CD63 (rs = -0.31, p < 0.001) and CD203c (rs = -0.35, p < 0.001). BAT to peanut gating basophils on CD203c+/CD123+/HLA-DR- reduced the false-negatives (1 vs. 5 %) and showed a higher diagnostic accuracy compared to using CD123+/HLA-DR- (97 vs. 91 %). CD203c+ appeared as an alternative gating strategy allowing two-colour BAT.
conclusionsBasophils of a subset of patients down-regulate CD123 with activation. The use of CD203c before gating on CD123+/HLA-DR- cells or in isolation ensures the identification of the entire basophil population and accurate assessment of basophil activation, with important diagnostic implications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.