ArticleActa pharmacologica Sinica2016
Hyaluronan suppresses lidocaine-induced apoptosis of human chondrocytes in vitro by inhibiting the p53-dependent mitochondrial apoptotic pathway.
Article in Acta pharmacologica Sinica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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18 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- Pooled it
- A clinical trial of ropivacaine in arthocentesis for TMD.BMC oral health · 2024Trial
- Effects of local anesthetics on yield and differentiation of synovial mesenchymal stem cells.Scientific reports · 2026Article
- Chondrotoxicity of Intra-Articular Injection Treatment: A Scoping Review.International journal of molecular sciences · 2024Article
- P53: A Key Target in the Development of Osteoarthritis.Molecular biotechnology · 2024Review
- Potential Mechanisms Underlying the Therapeutic Roles ofCurrent computer-aided drug design · 2024Article
- Exploring the wound healing potential of dietary nitrate in diabetic rat model.Frontiers in physiology · 2024Article
- Bone mesenchymal stem cell-derived exosomes involved co-delivery and synergism effect with icariin via mussel-inspired multifunctional hydrogel for cartilage protection.Asian journal of pharmaceutical sciences · 2023Article
- 3'UTR shortening of HAS2 promotes hyaluronan hyper-synthesis and bioenergetic dysfunction in pulmonary hypertension.Matrix biology : journal of the International Society for Matrix Biology · 2022Article
- Article
- Inhibition effect ofInternational journal of medical sciences · 2021Article
- Article
- Bioengineered tumor microenvironments with naked mole rats high-molecular-weight hyaluronan induces apoptosis in breast cancer cells.Oncogene · 2019Article
- Cytotoxicity of drugs injected into joints in orthopaedics.Bone & joint research · 2019Article
- Nobiletin Inhibits IL-1β-Induced Inflammation in ChondrocytesFrontiers in pharmacology · 2019Article
- Protective effects of platelet-rich plasma against lidocaine cytotoxicity on canine articular chondrocytes.Acta veterinaria Scandinavica · 2018Article
- Hyaluronan in the experimental injury of the cartilage: biochemical action and protective effects.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2018Review
- IMPROVEMENTS IN HIP OSTEOARTHRITIS WITH LAVAGE, TRIAMCINOLONE AND HYLAN G-F20.Acta ortopedica brasileiraArticle
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimIntra-articular injection of local anesthetics (LAs) is a common procedure for therapeutic purposes. However, LAs have been found toxic to articular cartilage, and hyaluronan may attenuate this toxicity. In this study we investigated whether hyaluronan attenuated lidocaine-induced chondrotoxicity, and if so, to elucidate the underlying mechanisms.
methodsHuman chondrocyte cell line SW1353 and newly isolated murine chondrocytes were incubated in culture medium containing hyaluronan and/or lidocaine for 72 h. Cell viability was evaluated using MTT assay. Cell apoptosis was detected with DAPI staining, caspase 3/7 activity assay and flow cytometry. Cell cycle distributions, ROS levels and mitochondrial membrane potential (ΔΨm) were determined using flow cytometry. The expression of p53 and p53-regulated gene products was measured with Western blotting.
resultsLidocaine (0.005%-0.03%) dose-dependently decreased the viability of SW1353 cells. This local anesthetic (0.015%, 0.025%) induced apoptosis, G2/M phase arrest and loss of ΔΨm, and markedly increased ROS production in SW1353 cells. Hyaluronan (50-800 μg/mL) alone did not affect the cell viability, but co-treatment with hyaluronan (200 μg/mL) significantly attenuated lidocaine-induced apoptosis and other abnormalities in SW1353 cells. Furthermore, co-treatment with lidocaine and hyaluronan significantly decreased the levels of p53 and its transcription targets Bax and p21 in SW1353 cells, although treatment with lidocaine alone did not significantly change these proteins. Similar results were obtained in ex vivo cultured murine chondrocytes.
conclusionHyaluronan suppresses lidocaine-induced apoptosis of human chondrocytes in vitro through inhibiting the p53-dependent mitochondrial apoptotic pathway.
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