ArticleScientific reports2016
Pado, a fluorescent protein with proton channel activity can optically monitor membrane potential, intracellular pH, and map gap junctions.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 44 citations in OpenAlex.
- Beyond Membrane Potential: Exploiting Signal Complexity in Genetically Encoded Voltage Indicators.Sensors (Basel, Switzerland) · 2026Article
- Simultaneous Whole-Cell Recording and Calcium Imaging Does Not Reveal Electrically Coupled Neurons ineNeuro · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Spatially Resolved Mapping of Voltage-Gated Proton Channel Activity Reveals Delayed Proton Transport in Local Microenvironments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Hydrophobic residues in S1 modulate enzymatic function and voltage sensing in voltage-sensing phosphatase.The Journal of general physiology · 2024Article
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Transcendent Aspects of Proton Channels.Annual review of physiology · 2024Review
- A new player in circadian networks: Role of electrical synapses in regulating functions of the circadian clock.Frontiers in physiology · 2022Review
- Conserved Amino Acids Residing Outside the Voltage Field Can Shift the Voltage Sensitivity and Increase the Signal Speed and Size ofFrontiers in cell and developmental biology · 2022Article
- Mechanism of ArcLight derived GEVIs involves electrostatic interactions that can affect proton wires.Biophysical journal · 2021Article
- Improving the flexibility of genetically encoded voltage indicators via intermolecular FRET.Biophysical journal · 2021Article
- Biophysical Parameters of GEVIs: Considerations for Imaging Voltage.Biophysical journal · 2020Review
- Optical consequences of a genetically-encoded voltage indicator with a pH sensitive fluorescent protein.Neuroscience research · 2019Review
- Imaging and analysis of genetically encoded calcium indicators linking neural circuits and behaviors.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2019Review
- Article
- Using Genetically Encoded Voltage Indicators (GEVIs) to Study the Input-Output Transformation of the Mammalian Olfactory Bulb.Frontiers in cellular neuroscience · 2019Article
- Optimizing Strategies for Developing Genetically Encoded Voltage Indicators.Frontiers in cellular neuroscience · 2019Review
- Engineering Photoactivatability in Genetically Encoded Voltage and pH Indicators.Frontiers in cellular neuroscience · 2019Article
- Comparative genomic analysis suggests that the sperm-specific sodium/proton exchanger and soluble adenylyl cyclase are key regulators of CatSper among the Metazoa.Zoological letters · 2019Article
- A dimeric fluorescent protein yields a bright, red-shifted GEVI capable of population signals in brain slice.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An in silico search strategy was developed to identify potential voltage-sensing domains (VSD) for the development of genetically encoded voltage indicators (GEVIs). Using a conserved charge distribution in the S2 α-helix, a single in silico search yielded most voltage-sensing proteins including voltage-gated potassium channels, voltage-gated calcium channels, voltage-gated sodium channels, voltage-gated proton channels, and voltage-sensing phosphatases from organisms ranging from mammals to bacteria and plants. A GEVI utilizing the VSD from a voltage-gated proton channel identified from that search was able to optically report changes in membrane potential. In addition this sensor was capable of manipulating the internal pH while simultaneously reporting that change optically since it maintains the voltage-gated proton channel activity of the VSD. Biophysical characterization of this GEVI, Pado, demonstrated that the voltage-dependent signal was distinct from the pH-dependent signal and was dependent on the movement of the S4 α-helix. Further investigation into the mechanism of the voltage-dependent optical signal revealed that inhibiting the dimerization of the fluorescent protein greatly reduced the optical signal. Dimerization of the FP thereby enabled the movement of the S4 α-helix to mediate a fluorescent response.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.