Evidence map›Paper›PMID 27040763›Full record

ArticleBiochemical and biophysical research communications2016

Focal adhesion kinase regulation in stem cell alignment and spreading on nanofibers.

Mohammad Nahid Andalib, Jeong Soon Lee, Ligyeom Ha, Yuris Dzenis, Jung Yul Lim

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.5field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 38 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
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  6. Article
  7. Modeling stem cell nucleus mechanics using confocal microscopy.Biomechanics and modeling in mechanobiology · 2021
    Article
  8. Structural design and environmental applications of electrospun nanofibers.Composites. Part A, Applied science and manufacturing · 2020
    Review
  9. Maneuvering the Migration and Differentiation of Stem Cells with Electrospun Nanofibers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020
    Review
  10. Review
  11. Article
  12. Review
  13. Article
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Mohammad Nahid Andalib *Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Jeong Soon Lee *Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Ligyeom HaDepartment of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Yuris DzenisDepartment of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Jung Yul LimDepartment of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
University of Nebraska–Lincoln · US

Funding

Optimized Stents for the Femoropopliteal ArteryR01HL125736 · NHLBI · UNIVERSITY OF NEBRASKA OMAHA · PI KAMENSKIY, ALEXEY, MACTAGGART, JASON · 2015 to 2024
$6.3M
NHLBI NIH HHS R01 HL125736
6 · The paper itself

Abstract

While electrospun nanofibers have demonstrated the potential for novel tissue engineering scaffolds, very little is known about the molecular mechanism of how cells sense and adapt to nanofibers. Here, we revealed the role of focal adhesion kinase (FAK), one of the key molecular sensors in the focal adhesion complex, in regulating mesenchymal stem cell (MSC) shaping on nanofibers. We produced uniaxially aligned and randomly distributed nanofibers from poly(l-lactic acid) to have the same diameters (about 130 nm) and evaluated MSC behavior on these nanofibers comparing with that on flat PLLA control. C3H10T1/2 murine MSCs exhibited upregulations in FAK expression and phosphorylation (pY397) on nanofibrous cultures as assessed by immunoblotting, and this trend was even greater on aligned nanofibers. MSCs showed significantly elongated and well-spread morphologies on aligned and random nanofibers, respectively. In the presence of FAK silencing via small hairpin RNA (shRNA), cell elongation length in the aligned nanofiber direction (cell major axis length) was significantly decreased, while cells still showed preferred orientation along the aligned nanofibers. On random nanofibers, MSCs with FAK-shRNA showed impaired cell spreading resulting in smaller cell area and higher circularity. Our study provides new data on how MSCs shape their morphologies on aligned and random nanofibrous cultures potentially via FAK-mediated mechanism.

Indexed as

NanofibersAnimalsCells, CulturedFocal Adhesion Protein-Tyrosine KinasesMesenchymal Stem CellsMiceFocal Adhesion Protein-Tyrosine KinasesAligned and random nanofibersCell alignment and spreadingFocal adhesion kinaseMesenchymal stem cellsshRNA

Identifiers

PMID27040763
PMCPMC4893025
OpenAlexW2327236515

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.