ArticleBiomaterials2016
Comparative effect of physicomechanical and biomolecular cues on zone-specific chondrogenic differentiation of mesenchymal stem cells.
Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
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- Nanocomposite Hydrogels: A Promising Approach for the Treatment of Degenerative Joint Diseases.Small science · 2024Article
- Bio-integrated scaffold facilitates large bone regeneration dominated by endochondral ossification.Bioactive materials · 2024Article
- T2 mapping for knee cartilage degeneration in young patients with mild symptoms.BMC medical imaging · 2022Article
- The role of mechanobiology in bone and cartilage model systems in characterizing initiation and progression of osteoarthritis.APL bioengineering · 2022Review
- Decellularized Articular Cartilage Microgels as Microcarriers for Expansion of Mesenchymal Stem Cells.Gels (Basel, Switzerland) · 2022Article
- Oxymatrine Protects Chondrocytes against IL-1Evidence-based complementary and alternative medicine : eCAM · 2022Article
- High-precision, gelatin-based, hybrid, bilayer scaffolds using melt electro-writing to repair cartilage injury.Bioactive materials · 2021Article
- In-situ stable injectable collagen-based hydrogels for cell and growth factor delivery.Materialia · 2021Article
- Advances and prospects in biomimetic multilayered scaffolds for articular cartilage regeneration.Regenerative biomaterials · 2020Review
- Recent Progress in 3D Printing of Elastic and High-Strength Hydrogels for the Treatment of Osteochondral and Cartilage Diseases.Frontiers in bioengineering and biotechnology · 2020Review
- Simultaneous tractography and elastography imaging of the zone-specific structural and mechanical responses in articular cartilage under compressive loading.Biomedical optics express · 2019Article
- Challenges for Natural Hydrogels in Tissue Engineering.Gels (Basel, Switzerland) · 2019Review
- Sequential Zonal Chondrogenic Differentiation of Mesenchymal Stem Cells in Cartilage Matrices.Tissue engineering. Part A · 2019Article
- The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells.Acta biomaterialia · 2017Article
- The Functional Response of Mesenchymal Stem Cells to Electron-Beam Patterned Elastomeric Surfaces Presenting Micrometer to Nanoscale Heterogeneous Rigidity.Advanced materials (Deerfield Beach, Fla.) · 2017Article
- From intricate to integrated: Biofabrication of articulating joints.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2017Review
- Devitalized Stem Cell Microsheets for Sustainable Release of Osteogenic and Vasculogenic Growth Factors and Regulation of Anti-Inflammatory Immune Response.Advanced biosystems · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Current tissue engineering approaches to regeneration of articular cartilage rarely restore the tissue to its normal state because the generated tissue lacks the intricate zonal organization of the native cartilage. Zonal regeneration of articular cartilage is hampered by the lack of knowledge for the relation between physical, mechanical, and biomolecular cues and zone-specific chondrogenic differentiation of progenitor cells. This work investigated in 3D the effect of TGF-β1, zone-specific growth factors, optimum matrix stiffness, and adding nanofibers on the expression of chondrogenic markers specific to the superficial, middle, and calcified zones of articular cartilage by the differentiating human mesenchymal stem cells (hMSCs). Growth factors included BMP-7, IGF-1, and hydroxyapatite (HA) for the superficial, middle, and calcified zones, respectively; optimum matrix stiffness was 80 kPa, 2.1 MPa, and 320 MPa; and nanofibers were aligned horizontal, random, and perpendicular to the gel surface. hMSCs with zone-specific cell densities were encapsulated in engineered hydrogels and cultured with or without TGF-β1, zone-specific growth factor, optimum matrix modulus, and fiber addition and cultured in basic chondrogenic medium. The expression of encapsulated cells was measured by mRNA, protein, and biochemical analysis. Results indicated that zone-specific matrix stiffness had a dominating effect on chondrogenic differentiation of hMSCs to the superficial and calcified zone phenotypes. Addition of aligned nanofibers parallel to the direction of gel surface significantly enhanced expression of Col II in the superficial zone chondrogenic differentiation of hMSCs. Conversely, biomolecular factor IGF-1 in combination with TGF-β1 had a dominating effect on the middle zone chondrogenic differentiation of hMSCs. Results of this work could potentially lead to the development of multilayer grafts mimicking the zonal organization of articular cartilage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.