ArticleCurrent protocols in molecular biology2016
Metabolomics by Gas Chromatography-Mass Spectrometry: Combined Targeted and Untargeted Profiling.
Article in Current protocols in molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 427 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
427 citing papers in PubMed, 2 syntheses or guidelines pooled it, 857 citations in OpenAlex.
- Diagnostic performance of volatile organic compounds analysis and electronic noses for detecting colorectal cancer: a systematic review and meta-analysis.Frontiers in oncology · 2024Pooled it
- Pooled it
- Impact of a whole food, plant-based diet on LDL-cholesterol and cardiovascular risk factors in adults with heterozygous familial hypercholesterolemia: a randomized, two-period, two-treatment, crossover, fully controlled feeding trial.Nature communications · 2026Trial
- Prebiotic Administration to Chronic Kidney Disease Patients Modifies Their Fecal Microbiome and Host Metabolism.Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation · 2026Trial
- Serum Metabolite Profiles in Adults With Abdominal Obesity in Response to Consuming 1 Avocado Daily for 6 Months: An Exploratory Analysis.Journal of the American Heart Association · 2026Trial
- Cardiometabolic and molecular adaptations to 6-month intermittent fasting in middle-aged men and women with overweight: secondary outcomes of a randomized controlled trial.Nature communications · 2025Trial
- Metabolic reprogramming in plant defense: linking signaling networks to metabolomics-driven insights.Plant signaling & behavior · 2026Review
- Bacterial microbiome and flavor metabolome shifts in traditional Hinggan league sauerkraut fermentation.Food chemistry. Molecular sciences · 2026Article
- Metabolic profiling of retinal organoids reveals conserved core metabolites and alterations in glycolytic and amino acid metabolism.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Comparative characterization of meat quality and volatile compounds in raw meat from Magang geese (Food chemistry: X · 2026Article
- Analytical methods and tools for studying inositol phosphates.FEBS letters · 2026Review
- Impact of blood contamination on hydrophilic metabolomics in human meningioma tissue.Analytical and bioanalytical chemistry · 2026Article
- Early pregnancy metabolomic signatures for child growth trajectories: a prospective metabolome-wide association study.The American journal of clinical nutrition · 2026Article
- Acute-phase serum metabolomics signatures for predicting post-stroke cognitive impairment after ischemic stroke: a prospective cohort study.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Metabolomic-Metabolite Profiling: Progressive Insight and Biochemical Pathway in Crude Oil Waste Sludge Co-Composting Bioremediation.Metabolites · 2026Article
- Increased nutrient diversity can induce loss of microbial diversity through enhanced resource uptake.Nature ecology & evolution · 2026Article
- Select plasma oxylipins as potential biomarkers of effective indomethacin treatment response in preterm infants with patent ductus arteriosus.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Metabolic adaptations of starving purple sea urchins (Strongylocentrotus purpuratus) surviving in the barrens.Scientific reports · 2026Article
- Article
- Metabolomic Profiling of Plasma in Children with Drug-Resistant Epilepsy: The Effect of the Ketogenic Diet on Energy and Amino Acid Pathways.Nutrients · 2026Article
367 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 2 countries.
Funding
Abstract
Gas chromatography-mass spectrometry (GC-MS)-based metabolomics is ideal for identifying and quantitating small-molecule metabolites (<650 Da), including small acids, alcohols, hydroxyl acids, amino acids, sugars, fatty acids, sterols, catecholamines, drugs, and toxins, often using chemical derivatization to make these compounds sufficiently volatile for gas chromatography. This unit shows how GC-MS-based metabolomics allows integration of targeted assays for absolute quantification of specific metabolites with untargeted metabolomics to discover novel compounds. Complemented by database annotations using large spectral libraries and validated standard operating procedures, GC-MS can identify and semiquantify over 200 compounds from human body fluids (e.g., plasma, urine, or stool) per study. Deconvolution software enables detection of more than 300 additional unidentified signals that can be annotated through accurate mass instruments with appropriate data processing workflows, similar to untargeted profiling using liquid chromatography-mass spectrometry. GC-MS is a mature technology that uses not only classic detectors (quadrupole) but also target mass spectrometers (triple quadrupole) and accurate mass instruments (quadrupole-time of flight). This unit covers sample preparation from mammalian samples, data acquisition, quality control, and data processing.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.