ArticleAngewandte Chemie (International ed. in English)2016
Mechanically Induced Catalytic Amplification Reaction for Readout of Receptor-Mediated Cellular Forces.
Article in Angewandte Chemie (International ed. in English), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Synthetic aptamer mechanoreceptors enable cell-specific force sensing and temporal control via DNA circuits.Nature communications · 2026Article
- Molecular Force Sensors for Biological Application.International journal of molecular sciences · 2024Review
- RAD-TGTs: high-throughput measurement of cellular mechanotype via rupture and delivery of DNA tension probes.Nature communications · 2023Article
- Recent developments in DNA-based mechanical nanodevices.Chemical communications (Cambridge, England) · 2022Review
- The magnitude of LFA-1/ICAM-1 forces fine-tune TCR-triggered T cell activation.Science advances · 2022Article
- Molecular Tension Probes to Quantify Cell-Generated Mechanical Forces.Molecules and cells · 2022Review
- Tug-of-war: molecular dynamometers against living cells for analyzing sub-piconewton interaction of a specific protein with the cell membrane.Chemical science · 2021Article
- Mechanically Triggered Hybridization Chain Reaction.Angewandte Chemie (International ed. in English) · 2021Article
- DNA-Based Microparticle Tension Sensors (μTS) for Measuring Cell Mechanics in Non-planar Geometries and for High-Throughput Quantification.Angewandte Chemie (International ed. in English) · 2021Article
- Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope.Nature communications · 2021Article
- EGFR activation attenuates the mechanical threshold for integrin tension and focal adhesion formation.Journal of cell science · 2020Article
- Enhanced Molecular Tension Sensor Based on Bioluminescence Resonance Energy Transfer (BRET).ACS sensors · 2020Article
- DNA Nanotechnology as an Emerging Tool to Study Mechanotransduction in Living Systems.Small (Weinheim an der Bergstrasse, Germany) · 2019Review
- Programmable Multivalent DNA-Origami Tension Probes for Reporting Cellular Traction Forces.Nano letters · 2018Article
- Molecular Tension Probes to Investigate the Mechanopharmacology of Single Cells: A Step toward Personalized Mechanomedicine.Advanced healthcare materials · 2018Article
- Light-Responsive Polymer Particles as Force Clamps for the Mechanical Unfolding of Target Molecules.Nano letters · 2018Article
- Platelet integrins exhibit anisotropic mechanosensing and harness piconewton forces to mediate platelet aggregation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Molecular Tension Probes for Imaging Forces at the Cell Surface.Accounts of chemical research · 2017Review
- Nanopatterned Extracellular Matrices Enable Cell-Based Assays with a Mass Spectrometric Readout.Nano letters · 2017Article
- Ratiometric Tension Probes for Mapping Receptor Forces and Clustering at Intermembrane Junctions.Nano letters · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Mechanics play a fundamental role in cell biology, but detecting piconewton (pN) forces is challenging because of a lack of accessible and high throughput assays. A mechanically induced catalytic amplification reaction (MCR) for readout of receptor-mediated forces in cells is described. Mechanically labile DNA duplexes presenting ligands are surface immobilized such that specific receptor forces denature the duplex and thus expose a blocked primer. Amplification of primers is achieved using an isothermal polymerization reaction and quantified by fluorescence readout. As a proof of concept, the assay was used to test the activity of a mechanomodulatory drug and integrin adhesion receptor antibodies. To the best of our knowledge, this is the first example of a catalytic reaction triggered in response to molecular piconewton forces. The MCR may transform the field of mechanobiology by providing a new facile tool to detect receptor specific mechanics with the convenience of the polymerase chain reaction (PCR).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.