Evidence map›Paper›PMID 27037847›Full record

ArticleChembiochem : a European journal of chemical biology2016

Multiplexed Imaging of Protein Phosphorylation on Membranes Based on Ti(IV) Functionalized Nanopolymers.

Anton Iliuk, Li Li, Michael Melesse, Mark C Hall, W Andy Tao

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Anton IliukDepartment of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA.
Li LiDepartment of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA.
Michael MelesseDepartment of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA.
Mark C HallDepartment of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA.
W Andy TaoDepartment of Biochemistry, Purdue University, 201 S University, West Lafayette, IN, 47907, USA. watao@purdue.edu.
Purdue University West Lafayette · USTymora Analytical Operations (United States) · US

Funding

Transgenic Mouse Core Facility Shared Resource (TMCF-SR)P30CA023168 · NCI · PURDUE UNIVERSITY WEST LAFAYETTE · PI John Tesmer · 1985 to 2026
$43.4M
New Proteomic Technologies for the Analysis of Tyrosine Kinase Signaling PathwaysR01GM088317 · NIGMS · PURDUE UNIVERSITY · PI TAO, W. ANDY · 2010 to 2014
$1.6M
A Proteomic Platform to identify and Validate Biomarkers in Metabolic Syndrome and Coronary Artery DiseaseR01GM111788 · NIGMS · PURDUE UNIVERSITY · PI TAO, W. ANDY · 2015 to 2017
$900k
Multiplexed detection and imaging of protein phosphorylation based on soluble nanopolymersR44CA200040 · NCI · TYMORA ANALYTICAL OPERATIONS, LLC · PI ILIUK, ANTON · 2015 to 2016
$897k
Multiplexed detection and imaging of protein phosphorylation based on soluble nanR43EB015809 · NIBIB · TYMORA ANALYTICAL OPERATIONS, LLC · PI ILIUK, ANTON · 2012 to 2012
$148k
NCI NIH HHS P30 CA023168NCI NIH HHS R44 CA200040NIBIB NIH HHS R43 EB015809NIGMS NIH HHS R01 GM088317NIGMS NIH HHS R01 GM111788
6 · The paper itself

Abstract

Accurate protein phosphorylation analysis reveals dynamic cellular signaling events not evident from protein expression levels. The most dominant biochemical assay, western blotting, suffers from the inadequate availability and poor quality of phospho-specific antibodies for phosphorylated proteins. Furthermore, multiplexed assays based on antibodies are limited by steric interference between the antibodies. Here we introduce a multifunctionalized nanopolymer for the universal detection of phosphoproteins that, in combination with regular antibodies, allows multiplexed imaging and accurate determination of protein phosphorylation on membranes.

Indexed as

AntibodiesBlotting, WesternChemistry Techniques, AnalyticalDendrimersElectrophoresis, Polyacrylamide GelFluorescent DyesNanostructuresPhosphoproteinsTitaniumAntibodiesDendrimersFluorescent DyesPAMAM StarburstPhosphoproteinsTitaniumantibodiesdendrimersmembranesmultiplexed analysisphosphoproteins

Identifiers

PMID27037847
PMCPMC4870103
OpenAlexW2334661254

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.