ArticleCardiovascular research2016
Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output.
Article in Cardiovascular research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Rbm20 antisense oligonucleotides alleviate diastolic dysfunction in a mouse model of cardiometabolic heart failure (HFpEF).Cardiovascular research · 2025Article
- An ovary-intact postmenopausal HFpEF mouse model; menopause is more than just estrogen deficiency.American journal of physiology. Heart and circulatory physiology · 2025Article
- Mechanical loading reveals an intrinsic cardiomyocyte stiffness contribution to diastolic dysfunction in murine cardiometabolic disease.The Journal of physiology · 2024Article
- Profiling the Biomechanical Responses to Workload on the Human Myocyte to Explore the Concept of Myocardial Fatigue and Reversibility: Rationale and Design of the POWER Heart Failure Study.Journal of cardiovascular translational research · 2024Article
- Microtubule destabilization with colchicine increases the work output of myocardial slices.Journal of molecular and cellular cardiology plus · 2024Article
- Myocardial Fatigue: a Mechano-energetic Concept in Heart Failure.Current cardiology reports · 2022Review
- RBM20Journal of molecular and cellular cardiology · 2022Article
- Shortening the thick filament by partial deletion of titin's C-zone alters cardiac function by reducing the operating sarcomere length range.Journal of molecular and cellular cardiology · 2022Article
- Cardiomyocyte Microtubules: Control of Mechanics, Transport, and Remodeling.Annual review of physiology · 2022Review
- Sarcomere length in the beating heart: Synchronicity is optional.The Journal of general physiology · 2022Article
- Mavacamten preserves length-dependent contractility and improves diastolic function in human engineered heart tissue.American journal of physiology. Heart and circulatory physiology · 2021Article
- Automatic detection of adult cardiomyocyte for high throughput measurements of calcium and contractility.PloS one · 2021Article
- Need for Speed: The Importance of Physiological Strain Rates in Determining Myocardial Stiffness.Frontiers in physiology · 2021Article
- Myocardial slices come to age: an intermediate complexity in vitro cardiac model for translational research.Cardiovascular research · 2020Review
- Phosphodiesterase 9a Inhibition in Mouse Models of Diastolic Dysfunction.Circulation. Heart failure · 2020Article
- Mechanical loading of isolated cardiac muscle with a real-time computed Windkessel model of the vasculature impedance.Physiological reports · 2019Article
- End-diastolic force pre-activates cardiomyocytes and determines contractile force: role of titin and calcium.The Journal of physiology · 2019Article
- Considerations for anFrontiers in pharmacology · 2019Review
- Length-Dependent Activation of Contractility and Ca-Transient Kinetics in Auxotonically Contracting Isolated Rat Ventricular Cardiomyocytes.Frontiers in physiology · 2019Article
- Maturation of Pluripotent Stem Cell-Derived Cardiomyocytes: a Critical Step for Drug Development and Cell Therapy.Journal of cardiovascular translational research · 2018Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsA single isolated cardiomyocyte is the smallest functional unit of the heart. Yet, all single isolated cardiomyocyte experiments have been limited by the lack of proper methods that could reproduce a physiological cardiac cycle. We aimed to investigate the contractile properties of a single cardiomyocyte that correctly mimic the cardiac cycle. METHODS AND
resultsBy adjusting the parameters of the feedback loop, using a suitably engineered feedback system and recording the developed force and the length of a single rat cardiomyocyte during contraction and relaxation, we were able to construct force-length (FL) relations analogous to the pressure-volume (PV) relations at the whole heart level. From the cardiac loop graphs, we obtained, for the first time, the power generated by one single cardiomyocyte.
conclusionHere, we introduce a new approach that by combining mechanics, electronics, and a new type optical force transducer can measure the FL relationship of a single isolated cardiomyocyte undergoing a mechanical loop that mimics the PV cycle of a beating heart.
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