ArticleApplied and environmental microbiology2016
An Efficient Method To Generate Gene Deletion Mutants of the Rapamycin-Producing Bacterium Streptomyces iranensis HM 35.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- The razABCD regulatory island links azalomycin F biosynthesis in Streptomyces iranensis to orsellinic acid production in the fungus Aspergillus nidulans.The Journal of biological chemistry · 2026Article
- Rapamycin Plays a Pivotal Role in the Potent Antifungal Activity Exhibited AgainstMicroorganisms · 2025Article
- Application of a replicative targetable vector system for difficult-to-manipulate streptomycetes.Applied microbiology and biotechnology · 2025Article
- Endophytic Streptomyces: an underexplored source with potential for novel natural drug discovery and development.Archives of microbiology · 2024Review
- Streptomyces alleviate abiotic stress in plant by producing pteridic acids.Nature communications · 2023Article
- Streptomyces polyketides mediate bacteria-fungi interactions across soil environments.Nature microbiology · 2023Article
- Extremophile Metal Resistance: Plasmid-Encoded Functions in Streptomyces mirabilis.Applied and environmental microbiology · 2022Article
- Redirection of acyl donor metabolic flux for lipopeptide A40926B0 biosynthesis.Microbial biotechnology · 2022Article
- Inducing Global Expression of Actinobacterial Biosynthetic Gene Clusters.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Enabling Efficient Genetic Manipulations in a Rare ActinomyceteFrontiers in microbiology · 2022Article
- Lichen-like association of Chlamydomonas reinhardtii and Aspergillus nidulans protects algal cells from bacteria.The ISME journal · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledStreptomyces iranensis HM 35 is an alternative rapamycin producer to Streptomyces rapamycinicus Targeted genetic modification of rapamycin-producing actinomycetes is a powerful tool for the directed production of rapamycin derivatives, and it has also revealed some key features of the molecular biology of rapamycin formation in S. rapamycinicus. The approach depends upon efficient conjugational plasmid transfer from Escherichia coli to Streptomyces, and the failure of this step has frustrated its application to Streptomyces iranensis HM 35. Here, by systematically optimizing the process of conjugational plasmid transfer, including screening of various media, and by defining optimal temperatures and concentrations of antibiotics and Ca(2+) ions in the conjugation media, we have achieved exconjugant formation for each of a series of gene deletions in S. iranensis HM 35. Among them were rapK, which generates the starter unit for rapamycin biosynthesis, and hutF, encoding a histidine catabolizing enzyme. The protocol that we have developed may allow efficient generation of targeted gene knockout mutants of Streptomyces species that are genetically difficult to manipulate. IMPORTANCE: The developed protocol of conjugational plasmid transfer from Escherichia coli to Streptomyces iranensis may allow efficient generation of targeted gene knockout mutants of other genetically difficult to manipulate, but valuable, Streptomyces species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.