ArticleGenes & development2016
Splicing-independent loading of TREX on nascent RNA is required for efficient expression of dual-strand piRNA clusters in Drosophila.
Article in Genes & development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 89 citations in OpenAlex.
- In thebioRxiv : the preprint server for biology · 2026Article
- piRNA direct the chromatin reader to its genomic targets.bioRxiv : the preprint server for biology · 2026Article
- AbioRxiv : the preprint server for biology · 2026Article
- Recurrent innovation of protein-protein interactions in the Drosophila piRNA pathway.The EMBO journal · 2026Article
- Rapid disassembly and Piwi-independent reassembly ofRNA (New York, N.Y.) · 2026Article
- Aub, Vasa, and Armi concentration in phase-separated nuage is dispensable for piRNA biogenesis and transposon silencing.RNA (New York, N.Y.) · 2026Article
- Binding of heterochromatin protein Rhino to a subset of piRNA clusters depends on a combination of two histone marks.Nature structural & molecular biology · 2025Article
- piRNA processing within non-membrane structures is governed by constituent proteins and their functional motifs.The FEBS journal · 2025Review
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- Global Co-regulatory Cross Talk Between mMolecular neurobiology · 2025Article
- Transcriptional regulation of the piRNA pathway by Ovo in animal ovarian germ cells.Genes & development · 2025Article
- piRNA Defense Against Endogenous Retroviruses.Viruses · 2024Review
- RNA helicase D1PAS1 resolves R-loops and forms a complex for mouse pachytene piRNA biogenesis required for male fertility.Nucleic acids research · 2024Article
- Review
- Stress Induced Activation of LTR Retrotransposons in theLife (Basel, Switzerland) · 2023Article
- Maternally inherited siRNAs initiate piRNA cluster formation.Molecular cell · 2023Article
- The absence of core piRNA biogenesis factors does not impact efficient transposon silencing in Drosophila.PLoS biology · 2023Article
- Article
- The histone demethylase Kdm3 prevents auto-immune piRNAs production inScience advances · 2023Article
- TheeLife · 2022Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The conserved THO/TREX (transcription/export) complex is critical for pre-mRNA processing and mRNA nuclear export. In metazoa, TREX is loaded on nascent RNA transcribed by RNA polymerase II in a splicing-dependent fashion; however, how TREX functions is poorly understood. Here we show that Thoc5 and other TREX components are essential for the biogenesis of piRNA, a distinct class of small noncoding RNAs that control expression of transposable elements (TEs) in the Drosophila germline. Mutations in TREX lead to defects in piRNA biogenesis, resulting in derepression of multiple TE families, gametogenesis defects, and sterility. TREX components are enriched on piRNA precursors transcribed from dual-strand piRNA clusters and colocalize in distinct nuclear foci that overlap with sites of piRNA transcription. The localization of TREX in nuclear foci and its loading on piRNA precursor transcripts depend on Cutoff, a protein associated with chromatin of piRNA clusters. Finally, we show that TREX is required for accumulation of nascent piRNA precursors. Our study reveals a novel splicing-independent mechanism for TREX loading on nascent RNA and its importance in piRNA biogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.