Evidence map›Paper›PMID 27036751›Full record

ReviewThe Review of scientific instruments2016

Invited Review Article: Pump-probe microscopy.

Martin C Fischer, Jesse W Wilson, Francisco E Robles, Warren S Warren

Abstract readReview
In one paragraph

Review in The Review of scientific instruments, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.

0numbers the graph read from it
0cells of the map it votes in
56citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

56 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Martin C FischerDepartment of Chemistry, Duke University, Durham, North Carolina 27708, USA.
Jesse W WilsonDepartment of Chemistry, Duke University, Durham, North Carolina 27708, USA.
Francisco E RoblesDepartment of Chemistry, Duke University, Durham, North Carolina 27708, USA.
Warren S WarrenDepartments of Chemistry, Biomedical Engineering, Physics, and Radiology, Duke University, Durham, North Carolina 27708, USA.

Funding

INTEGRATED CENTER FOR IN VIVO MICROSCOPYP41EB015897 · NIBIB · DUKE UNIVERSITY · PI JOHNSON, GEORGE ALLAN · 2012 to 2017
$7.4M
Improving Melanoma Diagnosis with Pump-Probe Optical ImagingR01CA166555 · NCI · DUKE UNIVERSITY · PI WARREN, WARREN S · 2013 to 2017
$1.6M
Multi-modality optical molecular imaging for melanoma tumor margin assessmentF32CA183204 · NCI · DUKE UNIVERSITY · PI ROBLES, FRANCISCO E · 2014 to 2016
$131k
Novel Techniques in Nonlinear Optical Microscopy for Improving Melanoma DiagnosisF32CA168497 · NCI · DUKE UNIVERSITY · PI WILSON, JESSE WILLIAM · 2013 to 2015
$129k
NCI NIH HHS F32 CA168497NCI NIH HHS F32CA168497NCI NIH HHS F32 CA183204NCI NIH HHS F32CA183204NCI NIH HHS R01 CA166555NCI NIH HHS R01CA166555NIBIB NIH HHS P41 EB015897NIBIB NIH HHS P41EB015897
6 · The paper itself

Abstract

Multiphoton microscopy has rapidly gained popularity in biomedical imaging and materials science because of its ability to provide three-dimensional images at high spatial and temporal resolution even in optically scattering environments. Currently the majority of commercial and home-built devices are based on two-photon fluorescence and harmonic generation contrast. These two contrast mechanisms are relatively easy to measure but can access only a limited range of endogenous targets. Recent developments in fast laser pulse generation, pulse shaping, and detection technology have made accessible a wide range of optical contrasts that utilize multiple pulses of different colors. Molecular excitation with multiple pulses offers a large number of adjustable parameters. For example, in two-pulse pump-probe microscopy, one can vary the wavelength of each excitation pulse, the detection wavelength, the timing between the excitation pulses, and the detection gating window after excitation. Such a large parameter space can provide much greater molecular specificity than existing single-color techniques and allow for structural and functional imaging without the need for exogenous dyes and labels, which might interfere with the system under study. In this review, we provide a tutorial overview, covering principles of pump-probe microscopy and experimental setup, challenges associated with signal detection and data processing, and an overview of applications.

Indexed as

PhotonsAnimalsHumansImage Processing, Computer-AssistedMicroscopyOptical Phenomena

Identifiers

PMID27036751
PMCPMC4798998

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.