ArticleFree radical biology & medicine2016
A Western diet induced NAFLD in LDLR(-/)(-) mice is associated with reduced hepatic glutathione synthesis.
Article in Free radical biology & medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Glutathione Biology in Neurodegenerative and Metabolic Diseases: Molecular Mechanisms, Pathophysiological Roles, and Therapeutic Perspectives.International journal of molecular sciences · 2026Review
- Metabolic dysfunction-associated steatotic liver disease and steatohepatitis-associated hepatocarcinoma preclinical models.Nature reviews. Gastroenterology & hepatology · 2026Review
- Article
- Antioxidant Intervention in NAFLD: Astaxanthin and Kokum Modulate Redox Status and Lysosomal Degradation.Molecules (Basel, Switzerland) · 2026Article
- Antioxidant Strategies for Age-Related Oxidative Damage in Dogs.Veterinary sciences · 2025Review
- An unbiased ranking of murine dietary models based on their proximity to human metabolic dysfunction-associated steatotic liver disease (MASLD).Nature metabolism · 2024Article
- Glutathione: Lights and Shadows in Cancer Patients.Biomedicines · 2023Review
- Acetaminophen-Induced Hepatotoxicity in Obesity and Nonalcoholic Fatty Liver Disease: A Critical Review.Livers · 2023Article
- Investigation of the Effect of Curcumin on Protein Targets in NAFLD Using Bioinformatic Analysis.Nutrients · 2022Article
- The Role of the Transsulfuration Pathway in Non-Alcoholic Fatty Liver Disease.Journal of clinical medicine · 2021Review
- Measuring acetyl-CoA and acetylated histone turnover in vivo: Effect of a high fat diet.Analytical biochemistry · 2021Article
- Target Deconvolution of Fenofibrate in Nonalcoholic Fatty Liver Disease Using Bioinformatics Analysis.BioMed research international · 2021Article
- A Review of Dietary (Phyto)Nutrients for Glutathione Support.Nutrients · 2019Review
- Impact of Supplementary Amino Acids, Micronutrients, and Overall Diet on Glutathione Homeostasis.Nutrients · 2019Review
- Allopurinol potentiates the hepatoprotective effect of metformin and vitamin E in fructose-induced fatty liver in rats.Clinical and experimental hepatology · 2019Article
- Metabolomic signatures of low birthweight: Pathways to insulin resistance and oxidative stress.PloS one · 2018Article
- Stable isotope-based flux studies in nonalcoholic fatty liver disease.Pharmacology & therapeutics · 2018Review
- Monitoring the synthesis of biomolecules using mass spectrometry.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Article
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
Oxidative stress plays a key role in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). Glutathione is the major anti-oxidant involved in cellular oxidative defense, however there are currently no simple non-invasive methods for assessing hepatic glutathione metabolism in patients with NAFLD. As a primary source of plasma glutathione, liver plays an important role in interorgan glutathione homeostasis. In this study, we have tested the hypothesis that measurements of plasma glutathione turnover could be used to assess the hepatic glutathione metabolism in LDLR(-/)(-) mice, a mouse model of diet-induced NAFLD. Mice were fed a standard low fat diet (LFD) or a high fat diet containing cholesterol (a Western type diet (WD)). The kinetics of hepatic and plasma glutathione were quantified using the (2)H2O metabolic labeling approach. Our results show that a WD leads to reduced fractional synthesis rates (FSR) of hepatic (25%/h in LFD vs. 18%/h in WD, P<0.05) and plasma glutathione (43%/h in LFD vs. 21%/h in WD, P<0.05), without any significant effect on their absolute production rates (PRs). WD-induced concordant changes in both hepatic and plasma glutathione turnover suggest that the plasma glutathione turnover measurements could be used to assess hepatic glutathione metabolism. The safety, simplicity, and low cost of the (2)H2O-based glutathione turnover approach suggest that this method has the potential for non-invasive probing of hepatic glutathione metabolism in patients with NAFLD and other diseases.
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