Evidence map›Paper›PMID 27036194›Full record

ArticleBMC veterinary research2016

In situ detection of GM1 and GM2 gangliosides using immunohistochemical and immunofluorescent techniques for auxiliary diagnosis of canine and feline gangliosidoses.

Moeko Kohyama, Akira Yabuki, Kenji Ochiai, Yuya Nakamoto, Kazuyuki Uchida, Daisuke Hasegawa, Kimimasa Takahashi, Hiroaki Kawaguchi, Masaya Tsuboi, Osamu Yamato

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Moeko KohyamaLaboratory of Clinical Pathology, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima-shi, Kagoshima, 890-0065, Japan.
Akira YabukiLaboratory of Clinical Pathology, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima-shi, Kagoshima, 890-0065, Japan.
Kenji OchiaiLaboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka-shi, Iwate, 020-8550, Japan.
Yuya NakamotoKyoto Animal Referral Medical Center, 208-4 Shin-arami, Tai, Kumiyama-cho, Kuse-gun, Kyoto, 613-0036, Japan.
Kazuyuki UchidaLaboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyou-ku, Tokyo, 113-8657, Japan.
Daisuke HasegawaDepartment of Veterinary Radiology, Nippon Veterinary and Life Science University, 1-7-1 Kyouman-chou, Musashino-shi, Tokyo, 180-8602, Japan.
Kimimasa TakahashiDepartment of Veterinary Pathology, Nippon Veterinary and Life Science University, 1-7-1 Kyouman-chou, Musashino-shi, Tokyo, 180-8602, Japan.
Hiroaki KawaguchiLaboratory of Veterinary Histopathology, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima-shi, Kagoshima, 890-0065, Japan.
Masaya TsuboiLaboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyou-ku, Tokyo, 113-8657, Japan.
Osamu YamatoLaboratory of Clinical Pathology, Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima-shi, Kagoshima, 890-0065, Japan. osam@vet.kagoshima-u.ac.jp.
Kagoshima University · JPNippon Veterinary and Life Science University · JPThe University of Tokyo · JPIwate University · JPKyoto Medical Center · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGM1 and GM2 gangliosidoses are progressive neurodegenerative lysosomal storage diseases resulting from the excessive accumulation of GM1 and GM2 gangliosides in the lysosomes, respectively. The diagnosis of gangliosidosis is carried out based on comprehensive findings using various types of specimens for histological, ultrastructural, biochemical and genetic analyses. Therefore, the partial absence or lack of specimens might have resulted in many undiagnosed cases. The aim of the present study was to establish immunohistochemical and immunofluorescent techniques for the auxiliary diagnosis of canine and feline gangliosidoses, using paraffin-embedded brain specimens stored for a long period.

resultsUsing hematoxylin and eosin staining, cytoplasmic accumulation of pale to eosinophilic granular materials in swollen neurons was observed in animals previously diagnosed with GM1 or GM2 gangliosidosis. The immunohistochemical and immunofluorescent techniques developed in this study clearly demonstrated the accumulated material to be either GM1 or GM2 ganglioside.

conclusionsImmunohistochemical and immunofluorescent techniques using stored paraffin-embedded brain specimens are useful for the retrospective diagnosis of GM1 and GM2 gangliosidoses in dogs and cats.

Indexed as

AnimalsBrainCat DiseasesCatsDog DiseasesDogsFluorescent Antibody TechniqueGangliosidosesG(M1) GangliosideG(M2) GangliosideImmunohistochemistryParaffin EmbeddingReproducibility of ResultsRetrospective StudiesG(M1) GangliosideG(M2) GangliosideCatDogGangliosidosisImmunofluorescenceImmunohistochemistryLysosomal Storage Disease

Identifiers

PMID27036194
PMCPMC4815186
OpenAlexW2319555100

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.