ArticleScientific reports2016
Development and application of a rapid and visual loop-mediated isothermal amplification for the detection of Sporisorium scitamineum in sugarcane.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- A comprehensive guide to loop-mediated isothermal amplification, an emerging diagnostic tool for plant pathogenic fungi.Frontiers in plant science · 2025Review
- Optimization of loop-mediated isothermal amplification assay for sunflower mildew disease detection.Scientific reports · 2024Article
- Fungal Diversity and Gibberellin Hormones Associated with Long Whips of Smut-Infected Sugarcanes.International journal of molecular sciences · 2024Article
- Sugarcane Smut: Current Knowledge and the Way Forward for Management.Journal of fungi (Basel, Switzerland) · 2021Review
- Proteomic Analysis of the Resistance Mechanisms in Sugarcane duringInternational journal of molecular sciences · 2019Article
- A Comparative Study of Three Detection Techniques forBioMed research international · 2018Article
- Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Ustilago maydis.Scientific reports · 2017Article
- Plant jasmonate ZIM domain genes: shedding light on structure and expression patterns of JAZ gene family in sugarcane.BMC genomics · 2017Article
- Small RNA sequencing reveals a role for sugarcane miRNAs and their targets in response to Sporisorium scitamineum infection.BMC genomics · 2017Article
- Comparative proteomics reveals that central metabolism changes are associated with resistance against Sporisorium scitamineum in sugarcane.BMC genomics · 2016Article
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8 authors.
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Abstract
Smut is a fungal disease with widespread prevalence in sugarcane planting areas. Early detection and proper identification of Sporisorium scitamineum are essential in smut management practices. In the present study, four specific primers targeting the core effector Pep1 gene of S. scitamineum were designed. Optimal concentrations of Mg(2+), primer and Bst DNA polymerase, the three important components of the loop-mediated isothermal amplification (LAMP) reaction system, were screened using a single factor experiment method and the L16(4(5)) orthogonal experimental design. Hence, a LAMP system suitable for detection of S. scitamineum was established. High specificity of the LAMP method was confirmed by the assay of S. scitamineum, Fusarium moniliforme, Pestalotia ginkgo, Helminthospcrium sacchari, Fusarium oxysporum and endophytes of Yacheng05-179 and ROC22. The sensitivity of the LAMP method was equal to that of the conventional PCR targeting Pep1 gene and was 100 times higher than that of the conventional PCR assay targeting bE gene in S. scitamineum. The results suggest that this novel LAMP system has strong specificity and high sensitivity. This method not only provides technological support for the epidemic monitoring of sugarcane smut, but also provides a good case for development of similar detection technology for other plant pathogens.
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