ArticleThe journal of physical chemistry letters2016
DEER Sensitivity between Iron Centers and Nitroxides in Heme-Containing Proteins Improves Dramatically Using Broadband, High-Field EPR.
Article in The journal of physical chemistry letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Roles of Three Conserved Active Site Residues of CytochromeJournal of the American Chemical Society · 2025Article
- Determining and controlling conformational information from orientationally selective light-induced triplet-triplet electron resonance spectroscopy for a set of bis-porphyrin rulers.Physical chemistry chemical physics : PCCP · 2024Article
- A personal account on 25 years of scientific literature on [FeFe]-hydrogenase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2023Review
- Orientation-Selective and Frequency-Correlated Light-Induced Pulsed Dipolar Spectroscopy.The journal of physical chemistry letters · 2021Article
- Strategies to identify and suppress crosstalk signals in double electron-electron resonance (DEER) experiments with gadoliniumMagnetic resonance (Gottingen, Germany) · 2020Article
- Pulsed EPR Dipolar Spectroscopy on Spin Pairs with one Highly Anisotropic Spin Center: The Low-Spin FeChemistry (Weinheim an der Bergstrasse, Germany) · 2019Article
- Light-Induced Pulsed EPR Dipolar Spectroscopy on a Paradigmatic Hemeprotein.Chemphyschem : a European journal of chemical physics and physical chemistry · 2019Article
- Enhancing sensitivity of Double Electron-Electron Resonance (DEER) by using Relaxation-Optimized Acquisition Length Distribution (RELOAD) scheme.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2019Article
- Site-Specific Incorporation of a CuThe journal of physical chemistry. B · 2018Article
- Integration of a versatile bridge concept in a 34 GHz pulsed/CW EPR spectrometer.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2018Article
- Monitoring Complex Formation by Relaxation-Induced Pulse Electron Paramagnetic Resonance Distance Measurements.Chemphyschem : a European journal of chemical physics and physical chemistry · 2017Article
- A Bifunctional Spin Label for Ligand Recognition on Surfaces.Angewandte Chemie (International ed. in English) · 2017Article
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10 authors.
Funding
Abstract
This work demonstrates the feasibility of making sensitive nanometer distance measurements between Fe(III) heme centers and nitroxide spin labels in proteins using the double electron-electron resonance (DEER) pulsed EPR technique at 94 GHz. Techniques to measure accurately long distances in many classes of heme proteins using DEER are currently strongly limited by sensitivity. In this paper we demonstrate sensitivity gains of more than 30 times compared with previous lower frequency (X-band) DEER measurements on both human neuroglobin and sperm whale myoglobin. This is achieved by taking advantage of recent instrumental advances, employing wideband excitation techniques based on composite pulses and exploiting more favorable relaxation properties of low-spin Fe(III) in high magnetic fields. This gain in sensitivity potentially allows the DEER technique to be routinely used as a sensitive probe of structure and conformation in the large number of heme and many other metalloproteins.
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