ArticlePLoS computational biology2016
A Well-Defined Readily Releasable Pool with Fixed Capacity for Storing Vesicles at Calyx of Held.
Article in PLoS computational biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Number and relative abundance of synaptic vesicles in functionally distinct priming states determine synaptic strength and short-term plasticity.The Journal of physiology · 2025Article
- Correction: A Well-Defined Readily Releasable Pool with Fixed Capacity for Storing Vesicles at Calyx of Held.PLoS computational biology · 2025Article
- Inferring synaptic transmission from the stochastic dynamics of the quantal content: An analytical approach.PLoS computational biology · 2025Article
- Estimates of quantal synaptic parameters in light of more complex vesicle pool models.Frontiers in cellular neuroscience · 2025Article
- The mitochondrial calcium uniporter inhibitor Ru265 increases neuronal excitability and reduces neurotransmission via off-target effects.British journal of pharmacology · 2024Article
- Exact Distribution of the Quantal Content in Synaptic Transmission.Physical review letters · 2024Article
- Parallel processing of quickly and slowly mobilized reserve vesicles in hippocampal synapses.eLife · 2024Article
- Complexin has a dual synaptic function as checkpoint protein in vesicle priming and as a promoter of vesicle fusion.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Interpretation of presynaptic phenotypes of synaptic plasticity in terms of a two-step priming process.The Journal of general physiology · 2024Article
- Article
- Developmental shift to mitochondrial respiration for energetic support of sustained transmission during maturation at the calyx of Held.Journal of neurophysiology · 2021Article
- Microtubule and Actin Differentially Regulate Synaptic Vesicle Cycling to Maintain High-Frequency Neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.eLife · 2019Article
- The Small and Dynamic Pre-primed Pool at the Release Site; A Useful Concept to Understand Release Probability and Short-Term Synaptic Plasticity?Frontiers in synaptic neuroscience · 2019Review
- Considerations for Measuring Activity-Dependence of Recruitment of Synaptic Vesicles to the Readily Releasable Pool.Frontiers in synaptic neuroscience · 2019Review
- Presynaptic GCaMP expression decreases vesicle release probability at the calyx of Held.Synapse (New York, N.Y.) · 2018Article
- Presynaptic loss of dynamin-related protein 1 impairs synaptic vesicle release and recycling at the mouse calyx of Held.The Journal of physiology · 2018Article
- Age-related defects in short-term plasticity are reversed by acetyl-L-carnitine at the mouse calyx of Held.Neurobiology of aging · 2018Article
- Degeneracy in the regulation of short-term plasticity and synaptic filtering by presynaptic mechanisms.The Journal of physiology · 2017Article
- The readily releasable pool of synaptic vesicles.Current opinion in neurobiology · 2017Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The readily releasable pool (RRP) of vesicles is a core concept in studies of presynaptic function. However, operating principles lack consensus definition and the utility for quantitative analysis has been questioned. Here we confirm that RRPs at calyces of Held from 14 to 21 day old mice have a fixed capacity for storing vesicles that is not modulated by Ca2+. Discrepancies with previous studies are explained by a dynamic flow-through pool, established during heavy use, containing vesicles that are released with low probability despite being immediately releasable. Quantitative analysis ruled out a posteriori explanations for the vesicles with low release probability, such as Ca2+-channel inactivation, and established unexpected boundary conditions for remaining alternatives. Vesicles in the flow-through pool could be incompletely primed, in which case the full sequence of priming steps downstream of recruitment to the RRP would have an average unitary rate of at least 9/s during heavy use. Alternatively, vesicles with low and high release probability could be recruited to distinct types of release sites; in this case the timing of recruitment would be similar at the two types, and the downstream transition from recruited to fully primed would be much faster. In either case, further analysis showed that activity accelerates the upstream step where vesicles are initially recruited to the RRP. Overall, our results show that the RRP can be well defined in the mathematical sense, and support the concept that the defining mechanism is a stable group of autonomous release sites.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.