ArticleBiotechnology for biofuels2016
Engineering of a high lipid producing Yarrowia lipolytica strain.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 170 citations in OpenAlex.
- EngineeringSynthetic and systems biotechnology · 2026Article
- Multi-omics reveals glucose repression of citric acid catabolism in Pichia kudriavzevii.Applied microbiology and biotechnology · 2025Article
- Regulating triacylglycerol cycling for high-efficiency production of polyunsaturated fatty acids and derivatives.Nature communications · 2025Article
- Where Biology Meets Engineering: Scaling Up Microbial Nutraceuticals to Bridge Nutrition, Therapeutics, and Global Impact.Microorganisms · 2025Review
- Model-Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production.Microbial biotechnology · 2025Article
- Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production.Microbial biotechnology · 2025Article
- Advances and perspectives in genetic expression and operation for the oleaginous yeastSynthetic and systems biotechnology · 2024Review
- Potential of oleaginous microbes for lipid accumulation and renewable energy generation.World journal of microbiology & biotechnology · 2024Review
- High-Efficiency Multiplexed Cytosine Base Editors for Natural Product Synthesis inACS synthetic biology · 2023Article
- Abolishing storage lipids induces protein misfolding and stress responses in Yarrowia lipolytica.Journal of industrial microbiology & biotechnology · 2023Article
- Identification of genome integration sites for developing a CRISPR-based gene expression toolkit in Yarrowia lipolytica.Microbial biotechnology · 2022Article
- Exploring Yeast Diversity to Produce Lipid-Based Biofuels from Agro-Forestry and Industrial Organic Residues.Journal of fungi (Basel, Switzerland) · 2022Review
- Lab-scale photobioreactor systems: principles, applications, and scalability.Bioprocess and biosystems engineering · 2022Review
- Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review.Biotechnology for biofuels and bioproducts · 2022Review
- Engineering of3 Biotech · 2022Article
- Zinc Finger Protein LipR Represses Docosahexaenoic Acid and Lipid Biosynthesis inApplied and environmental microbiology · 2022Article
- Brown seaweed hydrolysate as a promising growth substrate for biomass and lipid synthesis of the yeastFrontiers in bioengineering and biotechnology · 2022Article
- Production, Biosynthesis, and Commercial Applications of Fatty Acids From Oleaginous Fungi.Frontiers in nutrition · 2022Review
- The history, state of the art and future prospects for oleaginous yeast research.Microbial cell factories · 2021Review
- Establishment of genomic library technology mediated by non-homologous end joining mechanism in Yarrowia lipolytica.Science China. Life sciences · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrobial lipids are produced by many oleaginous organisms including the well-characterized yeast Yarrowia lipolytica, which can be engineered for increased lipid yield by up-regulation of the lipid biosynthetic pathway and down-regulation or deletion of competing pathways.
resultsWe describe a strain engineering strategy centered on diacylglycerol acyltransferase (DGA) gene overexpression that applied combinatorial screening of overexpression and deletion genetic targets to construct a high lipid producing yeast biocatalyst. The resulting strain, NS432, combines overexpression of a heterologous DGA1 enzyme from Rhodosporidium toruloides, a heterlogous DGA2 enzyme from Claviceps purpurea, and deletion of the native TGL3 lipase regulator. These three genetic modifications, selected for their effect on lipid production, enabled a 77 % lipid content and 0.21 g lipid per g glucose yield in batch fermentation. In fed-batch glucose fermentation NS432 produced 85 g/L lipid at a productivity of 0.73 g/L/h.
conclusionsThe yields, productivities, and titers reported in this study may further support the applied goal of cost-effective, large -scale microbial lipid production for use as biofuels and biochemicals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.