ArticleEvidence-based complementary and alternative medicine : eCAM2016
Cytotoxic and Apoptotic Activities of Methanolic Subfractions of Scrophularia oxysepala against Human Breast Cancer Cell Line.
Article in Evidence-based complementary and alternative medicine : eCAM, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
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- Synergistic inhibitory effects of the oxyresveratrol and dacarbazine combination against melanoma cells.Oncology letters · 2021Article
- Breast Cancer: A Global Concern, Diagnostic and Therapeutic Perspectives, Mechanistic Targets in Drug Development.Advanced pharmaceutical bulletin · 2021Review
- Middle Eastern Plant Extracts: An Alternative to Modern Medicine Problems.Molecules (Basel, Switzerland) · 2020Review
- In vitro anticancer activity ofEXCLI journal · 2020Article
- Antioxidant and Antigenotoxic Potential ofOxidative medicine and cellular longevity · 2020Article
- Phytochemical analysis and antiproliferative activity of the aerial parts ofResearch in pharmaceutical sciences · 2019Article
- Scrophularia Atropatana Extract Reverses TP53 Gene Promoter Hypermethylation and Decreases Survivin Antiapoptotic Gene Expression in Breast Cancer CellsAsian Pacific journal of cancer prevention : APJCP · 2018Article
- The genus Scrophularia: a source of iridoids and terpenoids with a diverse biological activity.Pharmaceutical biology · 2017Review
- Phytotherapy of nephrotoxicity-induced by cancer drugs: an updated review.Journal of nephropathology · 2017Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Herbs have played a positive role in medicine for thousands of years. In the current study, we investigated the cytotoxicity effects of Scrophularia oxysepala methanolic subfractions and the underlying mechanism responsible for cell death in human breast carcinoma (MCF-7 cells) and mouse fibrosarcoma (WEHI-164 cells). From 60% and 80% methanolic fractions, four subfractions (Fa, Fb, Fc, and Fd), yielded from size exclusion by Sephadex-LH20 column chromatography, were chosen. MTT assay revealed that all subfractions significantly reduced cell viability after 24 h and 36 h in a dose-dependent manner; it is worth noting that Fa and Fb subfractions had the highest cytotoxicity, with IC50 values of 52.9 and 61.2 μg/mL in MCF-7 at 24 h, respectively. ELISA, TUNEL, and DNA fragmentation assay revealed that antiproliferative effects of all subfractions were associated with apoptosis on cancer cells, without any significant effect on L929 normal cells. qRT-PCR data showed that, after 24 h treatment with IC50 concentrations of the subfractions, caspase-3 expression was increased in cancer cells while the expression of Bcl-2 was decreased. S. oxysepala methanolic subfractions induce apoptosis in MCF-7 and WEHI-164 cells and could be considered as a source of natural anticancer agents.
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Registered trials
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