ArticleProteome science2016
Investigation of proteome changes in osteoclastogenesis in low serum culture system using quantitative proteomics.
Article in Proteome science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 10 citations in OpenAlex.
- Effects of Lipid Metabolites and Cross Organ Lipid Metabolism Regulation on Bone Homeostasis.Aging and disease · 2025Review
- Production, Passaging Stability, and Histological Analysis of Madin-Darby Canine Kidney Cells Cultured in a Low-Serum Medium.Vaccines · 2024Article
- Novel Insights into Osteoclast Energy Metabolism.Current osteoporosis reports · 2023Review
- Orientin suppresses osteoclastogenesis and ameliorates ovariectomy-induced osteoporosis via suppressing ROS production.Food science & nutrition · 2023Article
- Thioacetamide promotes osteoclast transformation of bone marrow macrophages by influencing PI3K/AKT pathways.Journal of orthopaedic surgery and research · 2022Article
- Mitochondrial fatty acid β-oxidation is important for normal osteoclast formation in growing female mice.Frontiers in physiology · 2022Article
- The Role of Osteoclast Energy Metabolism in the Occurrence and Development of Osteoporosis.Frontiers in endocrinology · 2021Review
- Characterization of different osteoclast phenotypes in the progression of bone invasion by oral squamous cell carcinoma.Oncology reports · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRAW 264.7 cells can differentiate into osteoclasts when cultured in medium supplemented with 1 % FBS. However, the proteomic changes in the development of RAW 264.7 cells into osteoclasts in low serum culture system have not been elucidated. Therefore, we conducted quantitative proteomics analysis to investigate proteomic changes during osteoclastogenesis in low serum culture system.
resultsOur study confirmed that mature multinucleated osteoclasts were generated in a low serum culture system, validated by upregulated expression of 15 characteristic marker proteins, including TRAP, CTSK, MMP9, V-ATPase and ITGAV. Proteomics results demonstrated that 549 proteins expressed differentially in osteoclastogenesis in low serum culture system. In-depth bioinformatics analysis suggested that the differentially expressed proteins were mainly involved in mitochondrial activities and energy metabolism, including the electron transport chain pathway, TCA cycle pathway, mitochondrial LC-fatty acid beta-oxidation pathway and fatty acid biosynthesis pathway. The data have been deposited to the ProteomeXchange with identifier PXD001935.
conclusionOsteoclast formation is an ATP consuming procedure, whether occurring in a low serum culture system or a conventional culture system. In contrast to osteoclasts formed in conventional culture system, the fatty acid biosynthesis pathway was upregulated in osteoclasts cultured in low serum condition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.