ArticleJournal of experimental botany2016
Dominant and recessive mutations in the Raf-like kinase HT1 gene completely disrupt stomatal responses to CO2 in Arabidopsis.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 47 citations in OpenAlex.
- Guard cell photorespiration controls stomata behavior and development.The New phytologist · 2026Article
- Adjustments of plant primary metabolism in the face of climate change.Journal of experimental botany · 2025Review
- Stomatal CO2 sensing in plants: control of gas exchange and interactions with environmental stimuli.Plant & cell physiology · 2025Review
- Illuminating stomatal responses to red light: establishing the role of Ci-dependent versus -independent mechanisms in control of stomatal behaviour.Journal of experimental botany · 2024Article
- Starch metabolism in guard cells: At the intersection of environmental stimuli and stomatal movement.Plant physiology · 2024Review
- Stomatal CO2 responses at sub- and above-ambient CO2 levels employ different pathways in Arabidopsis.Plant physiology · 2024Article
- A network-based modeling framework reveals the core signal transduction network underlying high carbon dioxide-induced stomatal closure in guard cells.PLoS biology · 2024Article
- MPK12 in stomatal COThe New phytologist · 2023Article
- Wheat TaSnRK2.10 phosphorylates TaERD15 and TaENO1 and confers drought tolerance when overexpressed in rice.Plant physiology · 2023Article
- Stomatal COScience advances · 2022Article
- Plant hormone regulation of abiotic stress responses.Nature reviews. Molecular cell biology · 2022Review
- A serine-rich effector from the stripe rust pathogen targets a Raf-like kinase to suppress host immunity.Plant physiology · 2022Article
- Deep dive into CO2-dependent molecular mechanisms driving stomatal responses in plants.Plant physiology · 2021Article
- Article
- Elevated COPlants (Basel, Switzerland) · 2021Review
- ROS of Distinct Sources and Salicylic Acid Separate Elevated COFrontiers in plant science · 2020Article
- Mitogen-activated protein kinases MPK4 and MPK12 are key components mediating COThe Plant journal : for cell and molecular biology · 2018Article
- Abscisic acid-independent stomatal COProceedings of the National Academy of Sciences of the United States of America · 2018Article
- The BIG protein distinguishes the process of COThe New phytologist · 2018Article
- Blue light and CONature communications · 2017Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HT1 (HIGH LEAF TEMPERATURE 1) is the first component associated with changes in stomatal aperture in response to CO2 to be isolated by forward genetic screening. The HT1 gene encodes a protein kinase expressed mainly in guard cells. The loss-of-function ht1-1 and ht1-2 mutants in Arabidopsis thaliana have CO2-hypersensitive stomatal closure with concomitant reductions in their kinase activities in vitro In addition to these mutants, in this study we isolate or obtaine five new ht1 alleles (ht1-3, ht1-4, ht1-5, ht1-6, and ht1-7). Among the mutants, only ht1-3 has a dominant mutant phenotype and has widely opened stomata due to CO2 insensitivity. The ht1-3 mutant has a missense mutation affecting a non-conserved residue (R102K), whereas the other six recessive mutants have mutations in highly conserved residues in the catalytic domains required for kinase activity. We found that the dominant mutation does not affect the expression of HT1 or the ability to phosphorylate casein, a universal kinase substrate, but it does affect autophosphorylation activity in vitro A 3D structural model of HT1 also shows that the R102 residue protrudes from the surface of the kinase, implying a role for the formation of oligomers and/or interaction with its targets. We demonstrate that both the loss-of-function and gain-of-function ht1 mutants have completely disrupted CO2 responses, although they have normal responses to ABA. Furthermore, light-induced stomatal opening is smaller in ht1-3 and much smaller in ht1-2 Taken together, these results indicate that HT1 is a critical regulator for CO2 signaling and is partially involved in the light-induced stomatal opening pathway.
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