ArticleBiochimica et biophysica acta2016
Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvation.
Article in Biochimica et biophysica acta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Advances in ciliary proteomics - towards cracking the hidden proteome code of cilia.Journal of cell science · 2025Review
- The dynamic role of TRIM8, a novel ciliary protein, during various stages of mitosis.Cell death & disease · 2025Article
- The RNA splicing factor PRPF8 is required for left-right organiser cilia differentiation and determination of cardiac left-right asymmetry via regulation ofbioRxiv : the preprint server for biology · 2025Article
- Reversible and effective cell cycle synchronization method for studying stage-specific processes.Life science alliance · 2025Article
- CCP5 and CCP6 retain CP110 and negatively regulate ciliogenesis.BMC biology · 2023Article
- A targeted multi-proteomics approach generates a blueprint of the ciliary ubiquitinome.Frontiers in cell and developmental biology · 2023Article
- Cell cycle-related genes associate with sensitivity to hydrogen peroxide-induced toxicity.Redox biology · 2022Article
- Pathophysiology of Primary Cilia: Signaling and Proteostasis Regulation.Frontiers in cell and developmental biology · 2022Review
- Primary cilia and lipid raft dynamics.Open biology · 2021Review
- A CRISPR and high-content imaging assay compliant with ACMG/AMP guidelines for clinical variant interpretation in ciliopathies.Human genetics · 2021Article
- The Role of the U5 snRNP in Genetic Disorders and Cancer.Frontiers in genetics · 2021Review
- Targeting E3 Ubiquitin Ligases and Deubiquitinases in Ciliopathy and Cancer.International journal of molecular sciences · 2020Review
- Mutation spectrum of PRPF31, genotype-phenotype correlation in retinitis pigmentosa, and opportunities for therapy.Experimental eye research · 2020Review
- Freeing the brake: Proliferation needs primary cilium to disassemble.Journal of biosciences · 2020Review
- The deubiquitinating enzyme Usp14 controls ciliogenesis and Hedgehog signaling.Human molecular genetics · 2019Article
- The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia.Molecular biology of the cell · 2018Article
- Ganetespib limits ciliation and cystogenesis in autosomal-dominant polycystic kidney disease (ADPKD).FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- The centrosomal OFD1 protein interacts with the translation machinery and regulates the synthesis of specific targets.Scientific reports · 2017Article
- Mechanisms of ciliogenesis suppression in dividing cells.Cellular and molecular life sciences : CMLS · 2017Review
- Mitotic control by mRNA splicing regulators ensures primary cilia formation.Animal cells and systems · 2017Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Biogenesis of the primary cilium, a cellular organelle mediating various signaling pathways, is generally coordinated with cell cycle exit/re-entry. Although the dynamic cell cycle-associated profile of the primary cilium has been largely accepted, the mechanism governing the link between ciliogenesis and cell cycle progression has been poorly understood. Using a human genome-wide RNAi screen, we identify genes encoding subunits of the spliceosome and proteasome as novel regulators of ciliogenesis. We demonstrate that 1) the mRNA processing-related hits are essential for RNA expression of molecules acting in cilia disassembly, such as AURKA and PLK1, and 2) the ubiquitin-proteasome systems (UPS)-involved hits are necessary for proteolysis of molecules acting in cilia assembly, such as IFT88 and CPAP. In particular, we show that these screen hit-associated mechanisms are crucial for both cilia assembly and cell cycle arrest in response to serum withdrawal. Finally, our data suggest that the mRNA processing mechanism may modulate the UPS-dependent decay of cilia assembly regulators to control ciliary resorption-coupled cell cycle re-entry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.