ArticleScientific reports2016
4D (x-y-z-t) imaging of thick biological samples by means of Two-Photon inverted Selective Plane Illumination Microscopy (2PE-iSPIM).
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
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- Engineering Brain Organoids: Toward Mature Neural Circuitry with an Intact Cytoarchitecture.International journal of stem cells · 2022Review
- Identification of microplastics using 4-dimethylamino-4'-nitrostilbene solvatochromic fluorescence.Microscopy research and technique · 2021Article
- Review
- Effects of excitation light polarization on fluorescence emission in two-photon light-sheet microscopy.Biomedical optics express · 2020Article
- Current and future functional imaging techniques for post-traumatic stress disorder.RSC advances · 2019Review
- Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart.Journal of visualized experiments : JoVE · 2018Article
- Improved contrast in inverted selective plane illumination microscopy of thick tissues using confocal detection and structured illumination.Biomedical optics express · 2017Article
- Integrated single- and two-photon light sheet microscopy using accelerating beams.Scientific reports · 2017Article
- All-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide.Scientific reports · 2017Article
- Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets.Biophysical journal · 2016Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the last decade light sheet fluorescence microscopy techniques, such as selective plane illumination microscopy (SPIM), has become a well established method for developmental biology. However, conventional SPIM architectures hardly permit imaging of certain tissues since the common sample mounting procedure, based on gel embedding, could interfere with the sample morphology. In this work we propose an inverted selective plane microscopy system (iSPIM), based on non-linear excitation, suitable for 3D tissue imaging. First, the iSPIM architecture provides flexibility on the sample mounting, getting rid of the gel-based mounting typical of conventional SPIM, permitting 3D imaging of hippocampal slices from mouse brain. Moreover, all the advantages brought by two photon excitation (2PE) in terms of reduction of scattering effects and contrast improvement are exploited, demonstrating an improved image quality and contrast compared to single photon excitation. The system proposed represents an optimal platform for tissue imaging and it smooths the way to the applicability of light sheet microscopy to a wider range of samples including those that have to be mounted on non-transparent surfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.