ArticleScientific reports2016
Controlled rotation mechanism of DNA strand exchange by the Hin serine recombinase.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 9 citations in OpenAlex.
- Direct observation of subunit rotation during DNA strand exchange by serine recombinases.Nature communications · 2024Article
- Stick, stretch, and scan imaging method for DNA and filaments.RSC advances · 2021Article
- Force spectra of single bacterial amyloid CsgA nanofibers.RSC advances · 2020Article
- Snapshots of a molecular swivel in action.Nucleic acids research · 2018Article
- Torque and buckling in stretched intertwined double-helix DNAs.Physical review. E · 2017Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
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Abstract
DNA strand exchange by serine recombinases has been proposed to occur by a large-scale rotation of halves of the recombinase tetramer. Here we provide the first direct physical evidence for the subunit rotation mechanism for the Hin serine invertase. Single-DNA looping assays using an activated mutant (Hin-H107Y) reveal specific synapses between two hix sites. Two-DNA "braiding" experiments, where separate DNA molecules carrying a single hix are interwound, show that Hin-H107Y cleaves both hix sites and mediates multi-step rotational relaxation of the interwinding. The variable numbers of rotations in the DNA braid experiments are in accord with data from bulk experiments that follow DNA topological changes accompanying recombination by the hyperactive enzyme. The relatively slow Hin rotation rates, combined with pauses, indicate considerable rotary friction between synapsed subunit pairs. A rotational pausing mechanism intrinsic to serine recombinases is likely to be crucial for DNA ligation and for preventing deleterious DNA rearrangements.
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