ArticleInvestigative ophthalmology & visual science2016
Graft Site Microenvironment Determines Dendritic Cell Trafficking Through the CCR7-CCL19/21 Axis.
Article in Investigative ophthalmology & visual science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
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- IL-6 induces Treg dysfunction in desiccating stress-induced dry eye disease.Experimental eye research · 2024Article
- Role of Immune Cell Diversity and Heterogeneity in Corneal Graft Survival: A Systematic Review and Meta-Analysis.Journal of clinical medicine · 2021Review
- Age-Dependent Distinct Distributions of Dendritic Cells in Autoimmune Dry Eye Murine Model.Cells · 2021Article
- Ex Vivo-Induced Bone Marrow-Derived Myeloid Suppressor Cells Prevent Corneal Allograft Rejection in Mice.Investigative ophthalmology & visual science · 2021Article
- Topical administration of the kappa opioid receptor agonist nalfurafine suppresses corneal neovascularization and inflammation.Scientific reports · 2021Article
- Corneal Allografts: Factors for and against Acceptance.Journal of immunology research · 2021Review
- Plasmacytoid dendritic cells in the eye.Progress in retinal and eye research · 2021Review
- Variable Responses to Corneal Grafts: Insights from Immunology and Systems Biology.Journal of clinical medicine · 2020Review
- Newcastle Disease Virus Inhibits the Proliferation of T Cells Induced by Dendritic CellsFrontiers in immunology · 2020Article
- Regulatory T cell modulation of cytokine and cellular networks in corneal graft rejection.Current ophthalmology reports · 2018Article
- Therapeutic approaches for induction of tolerance and immune quiescence in corneal allotransplantation.Cellular and molecular life sciences : CMLS · 2018Review
- Method for selective quantification of immune and inflammatory cells in the cornea using flow cytometry.Journal of biological methods · 2018Article
- New insights into mononuclear phagocyte biology from the visual system.Nature reviews. Immunology · 2017Review
- Treatment of donor corneal tissue with immunomodulatory cytokines: a novel strategy to promote graft survival in high-risk corneal transplantation.Scientific reports · 2017Article
- Suppression of Neovascularization by Topical and Subconjunctival Bevacizumab After High-Risk Corneal Transplantation.Ophthalmology scienceArticle
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
purposeThe graft site microenvironment has a profound effect on alloimmunity and graft survival. We aimed to study the kinetics and phenotype of trafficking antigen-presenting cells (APC) to the draining lymph nodes (DLNs) in a mouse model of corneal transplantation, and to evaluate the homing mechanisms through which graft site inflammation controls APC trafficking.
methodsAllogeneic donor corneas were transplanted onto inflamed or quiescent graft beds. Host- (YAe+) and donor (CD45.1+ or eGFP+)-derived APCs were analyzed by flow cytometry. Protein and mRNA expression of the CC chemokine receptor (CCR)7 ligands CCL19 and CCL21 were assessed using ELISA and Real-Time qPCR, respectively. Transwell migration assay was performed to assess the effect of DLNs isolated from hosts with inflamed graft beds on mature bone marrow-derived dendritic cells (BMDCs).
resultsWe found that inflamed graft sites greatly promote the trafficking of both recipient- and graft-derived APCs, in particular mature CCR7+ CD11c+ dendritic cells (DC). CCL19 and CCL21 were expressed at significantly higher levels in the DLNs of recipients with inflamed graft beds. The supernatant of DLNs from recipients with inflamed graft beds induced a marked increase in mature DC migration compared with supernatant from recipients with quiescent graft beds in a transwell assay. This effect was abolished by neutralizing CCL19 or CCL21. These data suggest that graft site inflammation increases the expression of CCR7 ligands in the DLNs, which promote mature DC homing and allorejection.
conclusionsWe conclude that the graft site microenvironment plays a critical role in alloimmunity by determining DC trafficking through the CCR7-CCL19/21 axis.
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