ArticlePloS one2016
Addition of Cryoprotectant Significantly Alters the Epididymal Sperm Proteome.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Recent advances in cryotolerance biomarkers for semen preservation in frozen form-A systematic review.PloS one · 2024Pooled it
- Cryopreservation of Human Spermatozoa Using Penetrating Cryoprotectant-Free Vitrification Medium Exhibits an Altered Proteomic Profile Compared to the Conventional Rapid Freezing Method.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Physalis polysaccharide-sericin nanocarriers for controlled alpha-lipoic acid delivery and improved human sperm cryopreservation.Scientific reports · 2026Article
- Cryovial versus straw for sheep semen cryopreservation: a comparative study of surface area-to-volume ratio on post-thaw viability and in vitro embryo production.Scientific reports · 2026Article
- A comprehensive meta-analysis on the efficacy of growth factor enriched cryo-agents in enhancing post-thaw quality of mammalian semen.Animal bioscience · 2025Article
- ρ-Coumaric acid-zinc oxide nanoparticles improve post-thaw quality of goat spermatozoa and developmental competence of fertilized oocytes in vitro.Scientific reports · 2024Article
- Impact of various cryo-preservation steps on sperm rheotaxis and sperm kinematics in bull.Scientific reports · 2024Article
- Effect of fumigation height and time on cryopreservation of ram semen.Scientific reports · 2024Article
- A Pilot Analysis of Whole Transcriptome of Human Cryopreserved Sperm.International journal of molecular sciences · 2024Article
- High-throughput proteomic characterization of seminal plasma from bulls with contrasting semen quality.3 Biotech · 2023Article
- Hydrophobic soot nanoparticles as a non-cytotoxic motility activator of human spermatozoa.Nanoscale advances · 2022Article
- Cryoprotectants-Free Vitrification and Conventional Freezing of Human Spermatozoa: A Comparative Transcript Profiling.International journal of molecular sciences · 2022Article
- Evaluation of lipidomic change in goat sperm after cryopreservation.Frontiers in veterinary science · 2022Article
- Metabolomics analysis of buck semen cryopreserved with trehalose.Frontiers in genetics · 2022Article
- Effect of glycerol addition time on the cryopreserved Korean native brindle cattle (Chikso) sperm quality.Animal reproduction · 2022Article
- Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters.Animals : an open access journal from MDPI · 2021Article
- Unraveling Subcellular and Ultrastructural Changes During Vitrification of Human Spermatozoa: Effect of a Mitochondria-Targeted Antioxidant and a Permeable Cryoprotectant.Frontiers in cell and developmental biology · 2021Article
- Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality.International journal of molecular sciences · 2020Review
- Freezability biomarkers in bull epididymal spermatozoa.Scientific reports · 2019Article
- Human leukemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives.GigaScience · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although cryopreservation has been developed and optimized over the past decades, it causes various stresses, including cold shock, osmotic stress, and ice crystal formation, thereby reducing fertility. During cryopreservation, addition of cryoprotective agent (CPA) is crucial for protecting spermatozoa from freezing damage. However, the intrinsic toxicity and osmotic stress induced by CPA cause damage to spermatozoa. To identify the effects of CPA addition during cryopreservation, we assessed the motility (%), motion kinematics, capacitation status, and viability of epididymal spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining. Moreover, the effects of CPA addition were also demonstrated at the proteome level using two-dimensional electrophoresis. Our results demonstrated that CPA addition significantly reduced sperm motility (%), curvilinear velocity, viability (%), and non-capacitated spermatozoa, whereas straightness and acrosome-reacted spermatozoa increased significantly (p < 0.05). Ten proteins were differentially expressed (two decreased and eight increased) (>3 fold, p < 0.05) after CPA, whereas NADH dehydrogenase flavoprotein 2, f-actin-capping protein subunit beta, superoxide dismutase 2, and outer dense fiber protein 2 were associated with several important signaling pathways (p < 0.05). The present study provides a mechanistic basis for specific cryostresses and potential markers of CPA-induced stress. Therefore, these might provide information about the development of safe biomaterials for cryopreservation and basic ground for sperm cryopreservation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.