ArticleJournal of neurophysiology2016
DREADD-induced activation of subfornical organ neurons stimulates thirst and salt appetite.
Article in Journal of neurophysiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 62 citations in OpenAlex.
- Rapidly decreasing phasic dopamine responses precede a hedonic switch during satiation of sodium appetite.bioRxiv : the preprint server for biology · 2026Article
- The role of the prelimbic cortex to nucleus accumbens core projection in the reinstatement of cocaine-seeking after cocaine-alcohol polysubstance use.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026Article
- Neuropeptide Y modulates the electrical activity of subfornical organ neurons.Current research in neurobiology · 2025Article
- Control of sodium appetite by hindbrain aldosterone-sensitive neurons.Molecular and cellular endocrinology · 2024Review
- Dysregulation of the fluid homeostasis system by aging.bioRxiv : the preprint server for biology · 2024Article
- Magnetogenetic cell activation using endogenous ferritin.bioRxiv : the preprint server for biology · 2024Article
- Targeted stimulation of the vagus nerve reduces renal injury in female mice with systemic lupus erythematosus.Autonomic neuroscience : basic & clinical · 2023Article
- Chronic water restriction reduces sensitivity to brain stimulation reward in male and female rats.Physiology & behavior · 2023Article
- Sodium Intake and Disease: Another Relationship to Consider.Nutrients · 2023Review
- Sex Differences in Salt Appetite: Perspectives from Animal Models and Human Studies.Nutrients · 2023Review
- Ventral pallidum GABA neurons bidirectionally control opioid relapse across rat behavioral models.Addiction neuroscience · 2022Article
- Dopamine subsystems that track internal states.Nature · 2022Article
- Review
- Chronic Behavioral Manipulation via Orally Delivered Chemogenetic Actuator in Macaques.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Rational regulation of water-seeking effort in rodents.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Sensory Circumventricular Organs, Neuroendocrine Control, and Metabolic Regulation.Metabolites · 2021Review
- Pre-locus coeruleus neurons in rat and mouse.American journal of physiology. Regulatory, integrative and comparative physiology · 2021Article
- Thirst recruits phasic dopamine signaling through subfornical organ neurons.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Distinct CCK-positive SFO neurons are involved in persistent or transient suppression of water intake.Nature communications · 2020Article
- GJournal of neurophysiology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The subfornical organ (SFO) plays a pivotal role in body fluid homeostasis through its ability to integrate neurohumoral signals and subsequently alter behavior, neuroendocrine function, and autonomic outflow. The purpose of the present study was to evaluate whether selective activation of SFO neurons using virally mediated expression of Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) stimulated thirst and salt appetite. Male C57BL/6 mice (12-15 wk) received an injection of rAAV2-CaMKII-HA-hM3D(Gq)-IRES-mCitrine targeted at the SFO. Two weeks later, acute injection of clozapine N-oxide (CNO) produced dose-dependent increases in water intake of mice with DREADD expression in the SFO. CNO also stimulated the ingestion of 0.3 M NaCl. Acute injection of CNO significantly increased the number of Fos-positive nuclei in the SFO of mice with robust DREADD expression. Furthermore, in vivo single-unit recordings demonstrate that CNO significantly increases the discharge frequency of both ANG II- and NaCl-responsive neurons. In vitro current-clamp recordings confirm that bath application of CNO produces a significant membrane depolarization and increase in action potential frequency. In a final set of experiments, chronic administration of CNO approximately doubled 24-h water intake without an effect on salt appetite. These findings demonstrate that DREADD-induced activation of SFO neurons stimulates thirst and that DREADDs are a useful tool to acutely or chronically manipulate neuronal circuits influencing body fluid homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.