ArticleScientific reports2016
A High-Throughput Microfluidic Platform for Mammalian Cell Transfection and Culturing.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Sequence-defined polymers for predictable gene delivery.Nature reviews. Chemistry · 2026Review
- Platforms for Optogenetic Stimulation and Feedback Control.Frontiers in bioengineering and biotechnology · 2022Review
- Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications.Scientific reports · 2021Article
- High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology.Chemical reviews · 2021Review
- Testing Lab-on-a-Chip Technology for Culturing Human Melanoma Cells under Simulated Microgravity.Cancers · 2021Article
- Systematic Investigation of Insulin Fibrillation on a Chip.Molecules (Basel, Switzerland) · 2020Article
- A Disposable Passive Microfluidic Device for Cell Culturing.Biosensors · 2020Article
- Modular operation of microfluidic chips for highly parallelized cell culture and liquid dosing via a fluidic circuit board.Microsystems & nanoengineering · 2020Article
- A 'poly-transfection' method for rapid, one-pot characterization and optimization of genetic systems.Nucleic acids research · 2019Article
- High-Throughput Platform for Efficient Chemical Transfection, Virus Packaging, and Transduction.Micromachines · 2019Article
- A novel mammalian cell line development platform utilizing nanofluidics and optoelectro positioning technology.Biotechnology progress · 2018Article
- Calcium Imaging of GPCR Activation Using Arrays of Reverse Transfected HEK293 Cells in a Microfluidic System.Sensors (Basel, Switzerland) · 2018Article
- Study of the behavior of Euglena viridis, Euglena gracilis and Lepadella patella cultured in all-glass microaquarium.Biomedical microdevices · 2017Article
- Open-source, community-driven microfluidics with Metafluidics.Nature biotechnology · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian synthetic biology could be augmented through the development of high-throughput microfluidic systems that integrate cellular transfection, culturing, and imaging. We created a microfluidic chip that cultures cells and implements 280 independent transfections at up to 99% efficiency. The chip can perform co-transfections, in which the number of cells expressing each protein and the average protein expression level can be precisely tuned as a function of input DNA concentration and synthetic gene circuits can be optimized on chip. We co-transfected four plasmids to test a histidine kinase signaling pathway and mapped the dose dependence of this network on the level of one of its constituents. The chip is readily integrated with high-content imaging, enabling the evaluation of cellular behavior and protein expression dynamics over time. These features make the transfection chip applicable to high-throughput mammalian protein and synthetic biology studies.
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Registered trials
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