ArticleScientific reports2016
Quantitative non-invasive cell characterisation and discrimination based on multispectral autofluorescence features.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 99 citations in OpenAlex.
- Illuminating Immunity: A Systematic Review of Immune Cell Autofluorescence.Journal of biophotonics · 2025Pooled it
- Exfoliated kidney cells from urine for non-invasive kidney transplant monitoring: A potential opportunity?Clinical and experimental nephrology · 2026Review
- Automated Label-Free Classification of Circulating Tumor Cells and White Blood Cells Using Hyperspectral Imaging and Deep Learning on Microfluidic SACA Chip System.Micromachines · 2026Article
- Challenges and Prospects of Using Novel Nonlinear Effects in Multimode Optical Fibers for Multiphoton Endomicroscopy.Diagnostics (Basel, Switzerland) · 2026Review
- Article
- Integration of hyperspectral imaging and transcriptomics from individual cells with SpectralSeq.Genome research · 2025Article
- Multispectral autofluorescence for label free classification of immune cell type and activation/polarization status.Scandinavian journal of immunology · 2025Article
- Novel application of metabolic imaging of early embryos using a light-sheet on-a-chip device: a proof-of-concept study.Human reproduction (Oxford, England) · 2025Article
- Utilising Hyperspectral Autofluorescence Imaging in the Objective Assessment of Disease State and Pain in Patients with Rheumatoid Arthritis.International journal of molecular sciences · 2024Article
- Viewing early life without labels: optical approaches for imaging the early embryo†.Biology of reproduction · 2024Review
- Morphological Signal Processing for Phenotype Recognition of Human Pluripotent Stem Cells Using Machine Learning Methods.Biomedicines · 2023Article
- Article
- Volumetric live-cell autofluorescence imaging using Fourier light-field microscopy.Biomedical optics express · 2023Article
- Progress in Nano-Biosensors for Non-Invasive Monitoring of Stem Cell Differentiation.Biosensors · 2023Review
- Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams.Light, science & applications · 2022Article
- Exfoliated Kidney Cells from Urine for Early Diagnosis and Prognostication of CKD: The Way of the Future?International journal of molecular sciences · 2022Review
- Multispectral autofluorescence characteristics of reproductive aging in old and young mouse oocytes.Biogerontology · 2022Article
- Article
- Excitation-emission matrix fluorescence spectroscopy for cell viability testing in UV-treated cell culture.RSC advances · 2022Article
- Non-invasive assessment of exfoliated kidney cells extracted from urine using multispectral autofluorescence features.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Automated and unbiased methods of non-invasive cell monitoring able to deal with complex biological heterogeneity are fundamentally important for biology and medicine. Label-free cell imaging provides information about endogenous autofluorescent metabolites, enzymes and cofactors in cells. However extracting high content information from autofluorescence imaging has been hitherto impossible. Here, we quantitatively characterise cell populations in different tissue types, live or fixed, by using novel image processing and a simple multispectral upgrade of a wide-field fluorescence microscope. Our optimal discrimination approach enables statistical hypothesis testing and intuitive visualisations where previously undetectable differences become clearly apparent. Label-free classifications are validated by the analysis of Classification Determinant (CD) antigen expression. The versatility of our method is illustrated by detecting genetic mutations in cancer, non-invasive monitoring of CD90 expression, label-free tracking of stem cell differentiation, identifying stem cell subpopulations with varying functional characteristics, tissue diagnostics in diabetes, and assessing the condition of preimplantation embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.