ArticleScientific reports2016
Two-photon excited photoconversion of cyanine-based dyes.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- 1,3,2-Dioxaborine-based mero-polyanionic dyes: high-extinction NIR-absorbing polymethines.RSC advances · 2026Article
- Photophysics of Organic Fluorophore Photobluing and Its Applications in Fluorescence and Super-Resolution Microscopy.Accounts of chemical research · 2026Article
- Bitopic AJournal of medicinal chemistry · 2025Article
- Bleaching protection and axial sectioning in fluorescence nanoscopy through two-photon activation at 515 nm.Nature communications · 2024Article
- Thermal Truncation of Heptamethine Cyanine Dyes.Journal of the American Chemical Society · 2024Article
- Single Mesenchymal Stromal Cell Migration Tracking into Glioblastoma Using Photoconvertible Vesicles.Nanomaterials (Basel, Switzerland) · 2024Article
- Blue-shift photoconversion of near-infrared fluorescent proteins for labeling and tracking in living cells and organisms.Nature communications · 2023Article
- Dual-Color Photoconvertible Fluorescent Probes Based on Directed Photooxidation Induced Conversion for Bioimaging.Angewandte Chemie (International ed. in English) · 2023Article
- Defining the Basis of Cyanine Phototruncation Enables a New Approach to Single-Molecule Localization Microscopy.ACS central science · 2021Article
- Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging.Chemical science · 2021Article
- Photoblueing of organic dyes can cause artifacts in super-resolution microscopy.Nature methods · 2021Article
- Longitudinal monitoring of cancer cell subpopulations in monolayers, 3D spheroids, and xenografts using the photoconvertible dye DiR.Scientific reports · 2019Article
- Exploring organic photosensitizers based on hemicyanine derivatives: a sustainable approach for preparation of amide linkages.RSC advances · 2018Article
- Millisecond cellular labellingBiomedical optics express · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
The advent of phototransformable fluorescent proteins has led to significant advances in optical imaging, including the unambiguous tracking of cells over large spatiotemporal scales. However, these proteins typically require activating light in the UV-blue spectrum, which limits their in vivo applicability due to poor light penetration and associated phototoxicity on cells and tissue. We report that cyanine-based, organic dyes can be efficiently photoconverted by nonlinear excitation at the near infrared (NIR) window. Photoconversion likely involves singlet-oxygen mediated photochemical cleavage, yielding blue-shifted fluorescent products. Using SYTO62, a biocompatible and cell-permeable dye, we demonstrate photoconversion in a variety of cell lines, including depth-resolved labeling of cells in 3D culture. Two-photon photoconversion of cyanine-based dyes offer several advantages over existing photoconvertible proteins, including use of minimally toxic NIR light, labeling without need for genetic intervention, rapid kinetics, remote subsurface targeting, and long persistence of photoconverted signal. These findings are expected to be useful for applications involving rapid labeling of cells deep in tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.