ArticleScientific reports2016
Direct observation of intermediate states in model membrane fusion.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 20 citations in OpenAlex.
- Mechanistic Insights into Synaptotagmin-1 Mediated Membrane Fusion and Interactions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Forces of Change: Optical Tweezers in Membrane Remodeling Studies.The Journal of membrane biology · 2022Review
- Diversity of Extracellular Vesicles in Human Follicular Fluid: Morphological Analysis and Quantification.International journal of molecular sciences · 2022Article
- Membrane fusion studied by colloidal probes.European biophysics journal : EBJ · 2021Review
- Measuring Stepwise Binding of Thermally Fluctuating Particles to Cell Membranes without Fluorescence.Biophysical journal · 2020Article
- Formation of Cell Membrane Component Domains in Artificial Lipid Bilayer.Scientific reports · 2017Article
- SNARE-mediated membrane fusion trajectories derived from force-clamp experiments.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
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Abstract
We introduce a novel assay for membrane fusion of solid supported membranes on silica beads and on coverslips. Fusion of the lipid bilayers is induced by bringing an optically trapped bead in contact with the coverslip surface while observing the bead's thermal motion with microsecond temporal and nanometer spatial resolution using a three-dimensional position detector. The probability of fusion is controlled by the membrane tension on the particle. We show that the progression of fusion can be monitored by changes in the three-dimensional position histograms of the bead and in its rate of diffusion. We were able to observe all fusion intermediates including transient fusion, formation of a stalk, hemifusion and the completion of a fusion pore. Fusion intermediates are characterized by axial but not lateral confinement of the motion of the bead and independently by the change of its rate of diffusion due to the additional drag from the stalk-like connection between the two membranes. The detailed information provided by this assay makes it ideally suited for studies of early events in pure lipid bilayer fusion or fusion assisted by fusogenic molecules.
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