ArticleProteomics2016
Analysis of Drosophila melanogaster proteome dynamics during embryonic development by a combination of label-free proteomics approaches.
Article in Proteomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Drosophila melanogaster miPEP8 Regulates Cell Size Through its Interaction With ref(2)P/p62.Molecular & cellular proteomics : MCP · 2026Article
- A curated tissue-specific proteome, phosphoproteome, and kinome map of Drosophila melanogaster with an integrated outlook in circadian physiology.Functional & integrative genomics · 2025Article
- Differential regulation of the proteome and phosphoproteome along the dorso-ventral axis of the earlyeLife · 2024Article
- Article
- In Depth Exploration of the Alternative Proteome ofFrontiers in cell and developmental biology · 2022Article
- Label-free quantitative proteomics analysis of jujube (RSC advances · 2021Article
- Chemical Embryology Redux: Metabolic Control of Development.Trends in genetics : TIG · 2020Review
- Multi-level and lineage-specific interactomes of the Hox transcription factor Ubx contribute to its functional specificity.Nature communications · 2020Article
- Quantifying post-transcriptional regulation in the development of Drosophila melanogaster.Nature communications · 2018Article
- Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes.Drug metabolism and pharmacokinetics · 2018Article
- Spectral Libraries for SWATH-MS Assays for Drosophila melanogaster and Solanum lycopersicum.Proteomics · 2017Article
- The developmental proteome ofGenome research · 2017Article
- SWATH-MS dataset of heat-shock treatedData in brief · 2016Article
- SWATH-MS data ofData in brief · 2016Article
- Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
During embryogenesis, organisms undergo considerable cellular remodelling requiring the combined action of thousands of proteins. In case of the well-studied model Drosophila melanogaster, transcriptomic studies, most notably from the modENCODE project, have described in detail changes in gene expression at the mRNA level across development. Although such data are clearly very useful to understand how the genome is regulated during embryogenesis, it is important to understand how changes in gene expression are reflected at the level of the proteome. In this study, we describe a combination of two quantitative label-free approaches, SWATH and data-dependent acquisition, to monitor changes in protein expression across a timecourse of D. melanogaster embryonic development. We demonstrate that both approaches provide robust and reproducible methods for the analysis of proteome changes. In a preliminary analysis of Drosophila embryogenesis, we identified several pathways, including the heat-shock response, nuclear protein import and energy production that are regulated during embryo development. In some cases changes in protein expression mirrored transcript levels across development, whereas other proteins showed signatures of post-transcriptional regulation. Taken together, our pilot study provides a solid platform for a more detailed exploration of the embryonic proteome.
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Registered trials
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