ArticleBiophysical journal2016
Diagonally Scanned Light-Sheet Microscopy for Fast Volumetric Imaging of Adherent Cells.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Article
- Article
- Altair-LSFM A High-Resolution, Easy-to-Build Light-Sheet Microscope for Sub-Cellular Imaging.bioRxiv : the preprint server for biology · 2025Article
- Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D.iScience · 2025Article
- Morphological control of merlin-Rac antagonism in proliferation-promoting signaling.Science signaling · 2025Article
- Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM.The Journal of cell biology · 2025Article
- Compression-dependent microtubule reinforcement enables cells to navigate confined environments.Nature cell biology · 2024Article
- Transport and Organization of Individual Vimentin Filaments Within Dense Networks Revealed by Single Particle Tracking and 3D FIB-SEM.bioRxiv : the preprint server for biology · 2024Article
- u-track3D: Measuring, navigating, and validating dense particle trajectories in three dimensions.Cell reports methods · 2023Article
- Surface-guided computing to analyze subcellular morphology and membrane-associated signals in 3D.bioRxiv : the preprint server for biology · 2023Article
- Surface-guided computing to analyze subcellular morphology and membrane-associated signals in 3D.ArXiv · 2023Article
- Article
- Methods to Measure Water Permeability.Advances in experimental medicine and biology · 2023Article
- Fine-grained, nonlinear registration of live cell movies reveals spatiotemporal organization of diffuse molecular processes.PLoS computational biology · 2022Article
- In vivo 3D profiling of site-specific human cancer cell morphotypes in zebrafish.The Journal of cell biology · 2022Article
- Granger-causal inference of the lamellipodial actin regulator hierarchy by live cell imaging without perturbation.Cell systems · 2022Article
- Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm.International journal of molecular sciences · 2022Review
- Actin-Membrane Release Initiates Cell Protrusions.Developmental cell · 2020Article
- Super-resolution Microscopy with Single Molecules in Biology and Beyond-Essentials, Current Trends, and Future Challenges.Journal of the American Chemical Society · 2020Review
- A functionally neutral single chain antibody to measure beta-1 integrin uptake and recycling.Traffic (Copenhagen, Denmark) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
In subcellular light-sheet fluorescence microscopy (LSFM) of adherent cells, glass substrates are advantageously rotated relative to the excitation and emission light paths to avoid glass-induced optical aberrations. Because cells are spread across the sample volume, three-dimensional imaging requires a light-sheet with a long propagation length, or rapid sample scanning. However, the former degrades axial resolution and/or optical sectioning, while the latter mechanically perturbs sensitive biological specimens on pliant biomimetic substrates (e.g., collagen and basement membrane). Here, we use aberration-free remote focusing to diagonally sweep a narrow light-sheet along the sample surface, enabling multicolor imaging with high spatiotemporal resolution. Further, we implement a dithered Gaussian lattice to minimize sample-induced illumination heterogeneities, significantly improving signal uniformity. Compared with mechanical sample scanning, we drastically reduce sample oscillations, allowing us to achieve volumetric imaging at speeds of up to 3.5 Hz for thousands of Z-stacks. We demonstrate the optical performance with live-cell imaging of microtubule and actin cytoskeletal dynamics, phosphoinositide signaling, clathrin-mediated endocytosis, polarized blebbing, and endocytic vesicle sorting. We achieve three-dimensional particle tracking of clathrin-associated structures with velocities up to 4.5 μm/s in a dense intracellular environment, and show that such dynamics cannot be recovered reliably at lower volumetric image acquisition rates using experimental data, numerical simulations, and theoretical modeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.