ArticleScientific reports2016
Multiplexed Affinity-Based Separation of Proteins and Cells Using Inertial Microfluidics.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 72 citations in OpenAlex.
- Microparticle-enabled single cell multiparameter electronic immunophenotyping for selective electroporation.Lab on a chip · 2026Article
- A critical review of microfluidic electroporation for therapeutic cell engineering.Cell reports. Physical science · 2026Article
- Microfluidic Nanoparticle Separation for Precision Medicine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Blood component separation in straight microfluidic channels.Biomicrofluidics · 2023Article
- Label-free microfluidic isolation of functional and viable lymphocytes from peripheral blood mononuclear cells.Biomicrofluidics · 2023Article
- Article
- Red blood cell rosetting enables size-based separation of specific lymphocyte subsets from blood in a microfluidic device.Lab on a chip · 2023Article
- Inertial Microfluidics Enabling Clinical Research.Micromachines · 2021Review
- Microfluidic and Microscale Assays to Examine Regenerative Strategies in the Neuro Retina.Micromachines · 2020Review
- Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics.Lab on a chip · 2020Article
- 3D Printing of Inertial Microfluidic Devices.Scientific reports · 2020Article
- Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device.Micromachines · 2020Article
- Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using Acoustophoresis.Micromachines · 2019Article
- Spiral microfluidic devices for cell separation and sorting in bioprocesses.Biomicrofluidics · 2019Review
- Surface Modification of Glass/PDMS Microfluidic Valve Assemblies Enhances Valve Electrical Resistance.ACS applied materials & interfaces · 2019Article
- Rapid and Safe Isolation of Human Peripheral Blood B and T Lymphocytes through Spiral Microfluidic Channels.Scientific reports · 2019Article
- New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells.The Analyst · 2019Review
- Sustainable vaccine development: a vaccine manufacturer's perspective.Current opinion in immunology · 2018Review
- Manufacturing Cell Therapies Using Engineered Biomaterials.Trends in biotechnology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolation of low abundance proteins or rare cells from complex mixtures, such as blood, is required for many diagnostic, therapeutic and research applications. Current affinity-based protein or cell separation methods use binary 'bind-elute' separations and are inefficient when applied to the isolation of multiple low-abundance proteins or cell types. We present a method for rapid and multiplexed, yet inexpensive, affinity-based isolation of both proteins and cells, using a size-coded mixture of multiple affinity-capture microbeads and an inertial microfluidic particle sorter device. In a single binding step, different targets-cells or proteins-bind to beads of different sizes, which are then sorted by flowing them through a spiral microfluidic channel. This technique performs continuous-flow, high throughput affinity-separation of milligram-scale protein samples or millions of cells in minutes after binding. We demonstrate the simultaneous isolation of multiple antibodies from serum and multiple cell types from peripheral blood mononuclear cells or whole blood. We use the technique to isolate low abundance antibodies specific to different HIV antigens and rare HIV-specific cells from blood obtained from HIV+ patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.