ArticleScientific reports2016
Pitfalls in assessing microvascular endothelial barrier function: impedance-based devices versus the classic macromolecular tracer assay.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed.
- Evaluating the endothelial glycocalyx from bench to bedside: a translational framework for microvascular assessment.Angiogenesis · 2026Review
- vascr: An R-based toolkit for rapid and robust analysis of cellular impedance sensing data.European biophysics journal : EBJ · 2026Article
- Piezo1-Src signaling rapidly disrupts brain endothelial barrier integrity.Fluids and barriers of the CNS · 2026Article
- Stretchable Porous Membranes for Barrier Tissue Models with Real-Time Measurement and Biomimetic Cyclic Strain.Micromachines · 2025Article
- Faricimab Reverts VEGF-AInternational journal of molecular sciences · 2025Article
- Exploring Surface-Enhanced Raman Spectroscopy of Pyrazine-2-Carbonitrile for Indirect Label-Free Albumin Quantification in anAnalytical chemistry · 2025Article
- Novel Needle for Intravitreal Injections Does Not Affect Biological Activity of Anti-VEGF Drugs.Clinical ophthalmology (Auckland, N.Z.) · 2025Article
- The Protein Kinase A Inhibitor KT5720 Prevents Endothelial Dysfunctions Induced by High-Dose Irradiation.International journal of molecular sciences · 2024Article
- Amphetamine increases vascular permeability by modulating endothelial actin cytoskeleton and NO synthase via PAR-1 and VEGF-R.Scientific reports · 2024Article
- Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier.Journal of visualized experiments : JoVE · 2024Article
- Determination of Endothelial Barrier Resistance by Electric Cell-Substrate Impedance Sensing (ECIS) System.Methods in molecular biology (Clifton, N.J.) · 2024Article
- NanoIEA: A Nanopatterned Interdigitated Electrode Array-Based Impedance Assay for Real-Time Measurement of Aligned Endothelial Cell Barrier Functions.Advanced healthcare materials · 2024Article
- A ligand-independent Tie2-activating antibody reduces vascular leakage in models of Clarkson disease.Science advances · 2023Article
- A biomimetic chip to assess subcutaneous bioavailability of monoclonal antibodies in humans.PNAS nexus · 2023Article
- Alcohol and vascular endothelial function: Biphasic effect highlights the importance of dose.Alcohol, clinical & experimental research · 2023Article
- Blood-brain-barrier modeling with tissue chips for research applications in space and on Earth.Frontiers in space technologies · 2023Article
- High resolution monitoring of valvular interstitial cell driven pathomechanisms in procalcific environment using label-free impedance spectroscopy.Frontiers in cardiovascular medicine · 2023Article
- A decade of blood-brain barrier permeability assays: Revisiting old traumatic brain injury rat data for new insights and experimental design.Microvascular research · 2023Article
- Apoptosis signal-regulating kinase-1 regulates thrombin-induced endothelial permeability.Vascular pharmacology · 2022Article
- Beovu, but not Lucentis impairs the function of the barrier formed by retinal endothelial cells in vitro.Scientific reports · 2022Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The most frequently used parameters to describe the barrier properties of endothelial cells (ECs) in vitro are (i) the macromolecular permeability, indicating the flux of a macromolecular tracer across the endothelium, and (ii) electrical impedance of ECs grown on gold-film electrodes reporting on the cell layer's tightness for ion flow. Due to the experimental differences between these approaches, inconsistent observations have been described. Here, we present the first direct comparison of these assays applied to one single cell type (human microvascular ECs) under the same experimental conditions. The impact of different pharmacological tools (histamine, forskolin, Y-27632, blebbistatin, TRAP) on endothelial barrier function was analyzed by Transwell(®) tracer assays and two commercial impedance devices (xCELLigence(®), ECIS(®)). The two impedance techniques provided very similar results for all compounds, whereas macromolecular permeability readings were found to be partly inconsistent with impedance. Possible reasons for these discrepancies are discussed. We conclude that the complementary combination of both approaches is highly recommended to overcome the restrictions of each assay. Since the nature of the growth support may contribute to the observed differences, structure-function relationships should be based on cells that are consistently grown on either permeable or impermeable growth supports in all experiments.
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