ArticleNature communications2016
A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Unraveling the impact of miRNAs on gouty arthritis: diagnostic significance and therapeutic opportunities.Naunyn-Schmiedeberg's archives of pharmacology · 2025Review
- Article
- A new method to synthesize multiple gRNA libraries and functional mapping of mammalian H3K4me3 regions.Nucleic acids research · 2023Article
- Role of microRNA alternation in the pathogenesis of gouty arthritis.Frontiers in endocrinology · 2022Review
- A simple and rapid method for enzymatic synthesis of CRISPR-Cas9 sgRNA libraries.Nucleic acids research · 2021Article
- 5-Fluorouracil efficacy requires anti-tumor immunity triggered by cancer-cell-intrinsic STING.The EMBO journal · 2021Article
- CRISPR screen in cancer: status quo and future perspectives.American journal of cancer research · 2021Review
- BAHCC1 binds H3K27me3 via a conserved BAH module to mediate gene silencing and oncogenesis.Nature genetics · 2020Article
- CRISPR-Cas: a tool for cancer research and therapeutics.Nature reviews. Clinical oncology · 2019Review
- CRISPR/Cas9-mediated noncoding RNA editing in human cancers.RNA biology · 2018Review
- Role of the CRISPR system in controlling gene transcription and monitoring cell fate (Review).Molecular medicine reports · 2018Review
- Capture, amplification, and global profiling of microRNAs from low quantities of whole cell lysate.The Analyst · 2017Article
- Dense sgRNA Library Construction Using a Molecular Chipper Approach.Bio-protocol · 2017Article
- Novel determinants of mammalian primary microRNA processing revealed by systematic evaluation of hairpin-containing transcripts and human genetic variation.Genome research · 2017Article
- In vivo mutagenesis of miRNA gene families using a scalable multiplexed CRISPR/Cas9 nuclease system.Scientific reports · 2016Article
- A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism.Science advances · 2016Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Clustered regularly-interspaced palindromic repeats (CRISPR)-based genetic screens using single-guide-RNA (sgRNA) libraries have proven powerful to identify genetic regulators. Applying CRISPR screens to interrogate functional elements in noncoding regions requires generating sgRNA libraries that are densely covering, and ideally inexpensive, easy to implement and flexible for customization. Here we present a Molecular Chipper technology for generating dense sgRNA libraries for genomic regions of interest, and a proof-of-principle screen that identifies novel cis-regulatory domains for miR-142 biogenesis. The Molecular Chipper approach utilizes a combination of random fragmentation and a type III restriction enzyme to derive a densely covering sgRNA library from input DNA. Applying this approach to 17 microRNAs and their flanking regions and with a reporter for miR-142 activity, we identify both the pre-miR-142 region and two previously unrecognized cis-domains important for miR-142 biogenesis, with the latter regulating miR-142 processing. This strategy will be useful for identifying functional noncoding elements in mammalian genomes.
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