ArticleScientific reports2016
Transcriptional profiling analysis of Spodoptera litura larvae challenged with Vip3Aa toxin and possible involvement of trypsin in the toxin activation.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Establishment of antibodies-based novel multi-modal highly sensitive immunoassay platforms for detecting Bacillus thuringiensis Vip3Aa toxin in agricultural samples.Analytical and bioanalytical chemistry · 2026Article
- PxDorsal Regulates the Expression of Antimicrobial Peptides and Affects the Bt Susceptibility ofInsects · 2025Article
- N-Terminal α-Helices in Domain I ofToxins · 2024Article
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- Midgut serine proteinases participate in dietary adaptations of the castor (Eri) silkwormFrontiers in insect science · 2023Article
- Autophagy induced by Vip3Aa has a pro-survival role inVirulence · 2021Article
- Dynamics of Zea mays transcriptome in response to a polyphagous herbivore, Spodoptera litura.Functional & integrative genomics · 2021Article
- Response Mechanisms of Invertebrates toMicrobiology and molecular biology reviews : MMBR · 2021Review
- Dynamic changes of transcriptome of fifth-instar3 Biotech · 2021Article
- Vegetative Insecticidal Protein (Vip): A Potential Contender FromFrontiers in microbiology · 2021Review
- In silico identification of effector proteins from generalist herbivore Spodoptera litura.BMC genomics · 2020Article
- Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers.Toxins · 2020Review
- The Tripartite Interaction of Host Immunity-Toxins · 2020Review
- Article
- Identification of transcriptome and fluralaner responsive genes in the common cutworm Spodoptera litura Fabricius, based on RNA-seq.BMC genomics · 2020Article
- Activity of Vip3Aa1 againstOpen life sciences · 2020Article
- HT-SuperSAGE of the gut tissue of a Vip3Aa-resistant Heliothis virescens (Lepidoptera: Noctuidae) strain provides insights into the basis of resistance.Insect science · 2019Article
- Midgut Transcriptional Variation ofFrontiers in physiology · 2018Article
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Vip proteins, a new group of insecticidal toxins produced by Bacillus thuringiensis, are effective against specific pests including Spodoptera litura. Here, we report construction of a transcriptome database of S. litura by de novo assembly along with detection of the transcriptional response of S. litura larvae to Vip3Aa toxin. In total, 56,498 unigenes with an N50 value of 1,853 bp were obtained. Results of transcriptome abundance showed that Vip3Aa toxin provoked a wide transcriptional response of the S. litura midgut. The differentially expressed genes were enriched for immunity-related, metabolic-related and Bt-related genes. Twenty-nine immunity-related genes, 102 metabolic-related genes and 62 Bt-related genes with differential expression were found. On the basis of transcriptional profiling analysis, we focus on the functional validation of trypsin which potentially participated in the activation of Vip3Aa protoxin. Zymogram analysis indicated that the presence of many proteases, including trypsin, in S. litura larvae midgut. Results of enzymolysis in vitro of Vip3Aa by trypsin, and bioassay and histopathology of the trypsin-digested Vip3Aa toxin showed that trypsin was possibly involved in the Vip3Aa activation. This study provides a transcriptome foundation for the identification and functional validation of the differentially expressed genes in an agricultural important pest, S. litura.
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