Evidence map›Paper›PMID 27025227›Full record

ArticleLab on a chip2016

An embedded microretroreflector-based microfluidic immunoassay platform.

Balakrishnan Raja, Carmen Pascente, Jennifer Knoop, David Shakarisaz, Tim Sherlock, Steven Kemper, Katerina Kourentzi, Ronald F Renzi, Anson V Hatch, Juan Olano and 3 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Lab on a chip, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Balakrishnan RajaDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas, USA. willson@uh.edu.
Carmen PascenteDepartment of Electrical and Computer Engineering, University of Houston, Houston, Texas, USA.
Jennifer KnoopDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas, USA. willson@uh.edu.
David ShakarisazDepartment of Electrical and Computer Engineering, University of Houston, Houston, Texas, USA.
Tim SherlockDepartment of Electrical and Computer Engineering, University of Houston, Houston, Texas, USA.
Steven KemperDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas, USA. willson@uh.edu.
Katerina KourentziDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas, USA. willson@uh.edu.
Ronald F RenziAdvanced Systems Engineering and Deployment, Sandia National Laboratories, Livermore, California, USA.
Anson V HatchDepartment of Biotechnology and Bioengineering, Sandia National Laboratories, Livermore, California, USA.
Juan OlanoDepartment of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
Bi-Hung PengDepartment of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
Paul RuchhoeftDepartment of Electrical and Computer Engineering, University of Houston, Houston, Texas, USA.
Richard WillsonDepartment of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas, USA. willson@uh.edu and Centro de Biotecnología FEMSA, Tecnológico de Monterrey, Campus Monterrey, Monterrey, Nuevo León, Mexico.

Funding

WRCE Nonhuman Primate CoreU54AI057156 · NIAID · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI WALKER, DAVID H · 2003 to 2013
$102.6M
NIAID NIH HHS U54 AI057156
6 · The paper itself

Abstract

We present a microfluidic immunoassay platform based on the use of linear microretroreflectors embedded in a transparent polymer layer as an optical sensing surface, and micron-sized magnetic particles as light-blocking labels. Retroreflectors return light directly to its source and are highly detectable using inexpensive optics. The analyte is immuno-magnetically pre-concentrated from a sample and then captured on an antibody-modified microfluidic substrate comprised of embedded microretroreflectors, thereby blocking reflected light. Fluidic force discrimination is used to increase specificity of the assay, following which a difference imaging algorithm that can see single 3 μm magnetic particles without optical calibration is used to detect and quantify signal intensity from each sub-array of retroreflectors. We demonstrate the utility of embedded microretroreflectors as a new sensing modality through a proof-of-concept immunoassay for a small, obligate intracellular bacterial pathogen, Rickettsia conorii, the causative agent of Mediterranean Spotted Fever. The combination of large sensing area, optimized surface chemistry and microfluidic protocols, automated image capture and analysis, and high sensitivity of the difference imaging results in a sensitive immunoassay with a limit of detection of roughly 4000 R. conorii per mL.

Indexed as

Lab-On-A-Chip DevicesAnimalsAntibodies, ImmobilizedAutomation, LaboratoryCells, ImmobilizedComputer-Aided DesignEquipment DesignImage Processing, Computer-AssistedImmunoassayImmunomagnetic SeparationLimit of DetectionMagnetic PhenomenaMicroscopyMicroscopy, Electron, ScanningMicrospheresMicrotechnologyAntibodies, ImmobilizedPolymethyl Methacrylate

Identifiers

PMID27025227
PMCPMC5533084

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.