Evidence map›Paper›PMID 27023866›Full record

ArticlePloS one2016

Adiponectin at Physiologically Relevant Concentrations Enhances the Vasorelaxative Effect of Acetylcholine via Cav-1/AdipoR-1 Signaling.

Yunhui Du, Rui Li, Wayne Bigond Lau, Jianli Zhao, Bernard Lopez, Theodore A Christopher, Xin-Liang Ma, Yajing Wang

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Yunhui DuDepartment of Physiology and Pathophysiology, Capital Medical University, Beijing 100069, China.
Rui LiDepartment of Physiology, National Key Discipline and Key Laboratory of Cytophysiology of Shanxi Province, Shanxi Medical University, Shanxi, 030001, China.
Wayne Bigond LauDepartment of Emergency Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, United States of America.
Jianli ZhaoDepartment of Emergency Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, United States of America.
Bernard LopezDepartment of Emergency Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, United States of America.
Theodore A ChristopherDepartment of Emergency Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, United States of America.
Xin-Liang MaDepartment of Physiology and Pathophysiology, Capital Medical University, Beijing 100069, China.
Yajing WangDepartment of Physiology, National Key Discipline and Key Laboratory of Cytophysiology of Shanxi Province, Shanxi Medical University, Shanxi, 030001, China.
Thomas Jefferson University · USShanxi Medical University · CNCapital Medical University · CN

Funding

Targeting Adiponectin for Cardioprotection in the Ischemic HeartR01HL096686 · NHLBI · THOMAS JEFFERSON UNIVERSITY · PI MA, XIN-LIANG, WANG, YAJING · 2010 to 2024
$4.7M
Cav-3 in Diabetic Myocardial Injury Following Ischemia/ReperfusionR01HL123404 · NHLBI · THOMAS JEFFERSON UNIVERSITY · PI MA, XIN-LIANG · 2015 to 2023
$2.7M
Vevo 2100 ultrasound scannerS10OD010408 · OD · THOMAS JEFFERSON UNIVERSITY · PI FORSBERG, FLEMMING · 2012 to 2012
$525k
Cav-3 in Diabetic Myocardial Injury Following Ischemia/ReperfusionR56HL123404 · NHLBI · THOMAS JEFFERSON UNIVERSITY · PI MA, XIN-LIANG · 2014 to 2014
$388k
NHLBI NIH HHS HL-123404NHLBI NIH HHS HL-96686NHLBI NIH HHS R01 HL096686NHLBI NIH HHS R01 HL123404NHLBI NIH HHS R56 HL123404NIH HHS S10 OD010408
6 · The paper itself

Abstract

Clinical studies have identified hypoadiponectinemia as an independent hypertension risk factor. It is known that adiponectin (APN) can directly cause vasodilation, but the doses required exceed physiologic levels several fold. In the current study, we determine the effect of physiologically relevant APN concentrations upon vascular tone, and investigate the mechanism(s) responsible. Physiologic APN concentrations alone induced no significant vasorelaxation. Interestingly, pretreatment of wild type mouse aortae with physiologic APN levels significantly enhanced acetylcholine (ACh)-induced vasorelaxation (P<0.01), an endothelium-dependent and nitric oxide (NO)-mediated process. Knockout of adiponectin receptor 1 (AdipoR1) or caveolin-1 (Cav-1, a cell signaling facilitating molecule), but not adiponectin receptor 2 (AdipoR2) abolished APN-enhanced ACh-induced vasorelaxation. Immunoblot assay revealed APN promoted the AdipoR1/Cav1 signaling complex in human endothelial cells. Treatment of HUVECs with physiologic APN concentrations caused significant eNOS phosphorylation and nitric oxide (NO) production (P<0.01), an effect abolished in knockdown of either AdipoR1 or Cav-1. Taken together, these data demonstrate for the first time physiologic APN levels enhance the vasorelaxative response to ACh by inducing NO production through AdipoR1/Cav-1 mediated signaling. In physiologic conditions, APN plays an important function of maintaining vascular tone.

Indexed as

AcetylcholineAdiponectinAnimalsCaveolin 1Endothelium, VascularHumansHuman Umbilical Vein Endothelial CellsIn Vitro TechniquesMice, Inbred C57BLMice, KnockoutModels, BiologicalNitric OxideNitric Oxide Synthase Type IIIPhosphorylationReceptors, AdiponectinSignal TransductionAcetylcholineAdiponectinCaveolin 1Nitric OxideNitric Oxide Synthase Type IIIReceptors, Adiponectin

Identifiers

PMID27023866
PMCPMC4811582
OpenAlexW2326435950

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.