ArticleCytotechnology2016
Long term morphological characterization of mesenchymal stromal cells 3D spheroids built with a rapid method based on entry-level equipment.
Article in Cytotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Isolation, Expansion and Characterization of Mesenchymal Stromal Cells from Fresh and Cryopreserved Umbilical Cord Tissues in Horse and Dog.Animals : an open access journal from MDPI · 2026Article
- Free-floating long-term vascularized mesenchymal organoids.iScience · 2026Article
- Long-Term Culture of Cellular Spheroids in Novel Hydrogel Constructs for ECM Characterization in Bone Models.Materials (Basel, Switzerland) · 2025Article
- Equine Colostrum-Derived Mesenchymal Stromal Cells: A Potential Resource for Veterinary Regenerative Medicine.Veterinary sciences · 2025Article
- Current perspectives on the dynamic culture of mesenchymal stromal/stem cell spheroids.Stem cells translational medicine · 2025Review
- Synergistic Approach of High-Precision 3D Printing and Low Cell Adhesion for Enhanced Self-Assembled Spheroid Formation.Biosensors · 2024Article
- Optimization and Implication of Adipose-Derived Stem Cells in Craniofacial Bone Regeneration and Repair.Bioengineering (Basel, Switzerland) · 2024Review
- Mesenchymal Stem Cell Spheroids: A Promising Tool for Vascularized Tissue Regeneration.Tissue engineering and regenerative medicine · 2024Review
- Aggregation of human osteoblasts unlocks self-reliant differentiation and constitutes a microenvironment for 3D-co-cultivation with other bone marrow cells.Scientific reports · 2024Article
- Brain Homogenate of a Rat Model of Alzheimer's Disease Modifies the Secretome of 3D Cultured Periodontal Ligament Stem Cells: A Potential Neuroregenerative Therapy.Iranian journal of pharmaceutical research : IJPR · 2022Article
- Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment.Journal of visualized experiments : JoVE · 2022Article
- Novel Mesenchymal Stem Cell Spheroids with Enhanced Stem Cell Characteristics and Bone Regeneration Ability.Stem cells translational medicine · 2022Article
- Three-Dimensional Cell Culture Models to Study Respiratory Virus Infections Including COVID-19.Biomimetics (Basel, Switzerland) · 2021Review
- Characterization and quantification of necrotic tissues and morphology in multicellular ovarian cancer tumor spheroids using optical coherence tomography.Biomedical optics express · 2021Article
- The Duo of Osteogenic and Angiogenic Differentiation in ADSC-Derived Spheroids.Frontiers in cell and developmental biology · 2021Article
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- Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties.Stem cell reviews and reports · 2020Review
- A comparative study of the capacity of mesenchymal stromal cell lines to form spheroids.PloS one · 2020Article
- Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells.BMC veterinary research · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three-dimensional (3D) spheroids of mesenchymal stromal cells (MSC) have been demonstrated to improve a wide range of MSC features, such as multilineage potential, secretion of therapeutic factors, and resistance against hypoxic condition. Accordingly, they represent a promising tool in regenerative medicine for several biological and clinical applications. Many approaches have been proposed to generate MSC spheroids. They usually require specific generation systems, such as rotatory bioreactors or low-attachment plates, and each approach has its own disadvantages. Furthermore, an over-time analysis of morphological homogeneity and architectural stability of the spheroids generated is rarely provided. In this work we adapted the "pellet culture" method to obtain homogenous spheroids of MSC and maintain them in vitro for long term studies. We analysed their outer and inner structure over a 2-month period to provide morphological and architectural information regarding the spheroids generated. Quantitative and qualitative data were obtained using brightfield and confocal microscope imaging coupled to a computational analysis to estimate volume, sphericity, and jagging degree. In addition, histological evaluation was performed to more thoroughly assess the cellular composition and the internal architecture of the 3D spheroids. The results provided show that MSC spheroids generated with the proposed approach are homogeneous and stable, from both morphological and architectural points of view, for a period of at least 15 days, approximately between day 15 and day 30 after their generation. Accordingly, the approach proposed serves as a rapid, cost-effective, and efficient method to generate and maintain MSC spheroids using common entry-level laboratory equipment only.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.