Evidence map›Paper›PMID 27022787›Full record

ArticleRevista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia2016

High Risk HPV E6/E7 Oncoprotein Expression in Women with High Grade Squamous Intraepithelial Lesion.

Jefferson Elias Cordeiro Valença, Ana Katherine Gonçalves, Ismael Dale Cotrim Guerreiro da Silva, José Eleutério Junior, Terezinha Tenório da Silva, Danyelly Bruneska, Ricardo Arraes de Alencar Ximenes

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 1 country.

Jefferson Elias Cordeiro ValençaGynecology and Obstetric Department, Universidade Federal de Pernambuco - UFPE, Recife, PE, Brazil.
Ana Katherine GonçalvesGynecology and Obstetric Department, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
Ismael Dale Cotrim Guerreiro da SilvaGynecology and Obstetric Department, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
José Eleutério JuniorGynecology and Obstetric Department, Universidade Federal do Ceará, Fortaleza, CE, Brazil.
Terezinha Tenório da SilvaGynecology and Obstetric Department, Universidade Federal de Pernambuco - UFPE, Recife, PE, Brazil.
Danyelly BruneskaBiochemistry Department, UFPE, Recife, PE, Brazil.
Ricardo Arraes de Alencar XimenesTropical Medicine Department, UFPE, Recife, PE, Brazil.
Hospital das Clínicas da Universidade Federal de Pernambuco · BRUniversidade Federal de Pernambuco · BRUniversidade Federal de São Paulo · BRUniversidade Federal do Ceará · BRUniversidade Federal do Rio Grande do Norte · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo correlate the expression of high-risk HPV E6 mRNA with pap smear, colposcopy, and biopsy results in women with high grade squamous intraepithelial lesion (HSIL).

methodsA cross-sectional study was performed on women referred for primary care services after cytological diagnosis of HSIL. We evaluated the expression of E6/E7 mRNA of HPV types 16,18,31,33, and 45 and correlated the results with those of Pap smear, colposcopy, and biopsy. For amplification/detection of mRNA E6 / E7 we used NucliSENSEasyQ kit to detect HPV mRNA by polymerase chain reaction with primers/probes for HPV types 16, 18, 31, 33, and 45.

resultsOut of 128 valid tests, the results of 30 (23.4%) tests were negative and 98 (70%) tests were positive. Only one type of HPV was detected in 87.7% of the E6/E7 mRNA positive cases. HPV16 was detected in 61.2% of the cases, followed by HPV33 (26.5%), HPV31 (17.3%), HPV18 (10%), and HPV45 (4.08%). Pap smear tests revealed that the E6/E7 test was positive in 107 (83.8%) women with atypical squamous cells - high grade (ASC-H), HSIL, or higher. The E6/E7 test was positive in 69 (57.5%) specimens presenting negative cytology results. When analyzing the association with colposcopy results, the frequency of positive E6/E7 results increased with the severity of the injury, ranging from 57.1% in women without colposcopy-detected injury to 86.5% in those with higher levels of colposcopy findings. Of the 111 women who underwent biopsy and E6/E7 testing, the E6/E7 test was positive in 84.7% of the women who presented with lesions of cervical intraepithelial neoplasia (CIN) grade 2 or higher. Finally, 41.2% of women with a negative biopsy presented a positive E6/E7 test.

conclusionsE6/E7 mRNA expression was higher in women with HSIL and CIN grade 2 or higher.

Indexed as

AdultCross-Sectional StudiesFemaleHumansOncogene ProteinsOncogene Proteins, ViralPapillomaviridaePapillomavirus InfectionsPregnancyRNA, MessengerSquamous Intraepithelial Lesions of the CervixUterine Cervical DysplasiaUterine Cervical NeoplasmsVaginal SmearsOncogene ProteinsOncogene Proteins, ViralRNA, Messenger

Identifiers

PMID27022787
PMCPMC10309481
OpenAlexW2333195330

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.