Evidence map›Paper›PMID 27022629›Full record

ArticleeNeuro

Targeted Deletion of Vesicular GABA Transporter from Retinal Horizontal Cells Eliminates Feedback Modulation of Photoreceptor Calcium Channels.

Arlene A Hirano, Xue Liu, Jim Boulter, James Grove, Luis Pérez de Sevilla Müller, Steven Barnes, Nicholas C Brecha

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eNeuro. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 45 citations in OpenAlex.

  1. Article
  2. Review
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  4. Review
  5. Article
  6. Article
  7. T-Type CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2022
    Article
  8. AMIGO1 Promotes Axon Growth and Territory Matching in the Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022
    Article
  9. Article
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  15. Review
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  18. Article
  19. Review
  20. Vesicular GABA Transporter Is Necessary for Transplant-Induced Critical Period Plasticity in Mouse Visual Cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Arlene A HiranoDepartment of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095; Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, California 90073.ORCID 0000-0001-8842-3582
Xue LiuDepartment of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles , Los Angeles, California 90095.
Jim BoulterDepartment of Psychiatry and Biobehavioral Sciences, Hatos Research Center for Neuropharmacology, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles , Los Angeles, California 90095.
James GroveDepartment of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles , Los Angeles, California 90095.
Luis Pérez de Sevilla MüllerDepartment of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles , Los Angeles, California 90095.
Steven BarnesDepartment of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095; Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, California 90073; Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada; Department of Ophthalmology & Visual Sciences, Dalhousie University, Halifax, Nova Scotia B3H 4R2, Canada.
Nicholas C BrechaDepartment of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095; Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, California 90073; Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095; Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095.
University of California, Los Angeles · USDalhousie University · CA

Funding

Transmitter Release From Mammalian Horizontal Cells.R01EY015573 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BRECHA, NICHOLAS C. · 2005 to 2017
$4.9M
BLRD VA IK6 BX005230CIHR MOP10968NEI NIH HHS EY-15573NEI NIH HHS R01 EY015573
6 · The paper itself

Abstract

The cellular mechanisms underlying feedback signaling from horizontal cells to photoreceptors, which are important for the formation of receptive field surrounds of early visual neurons, remain unsettled. Mammalian horizontal cells express a complement of synaptic proteins that are necessary and sufficient for calcium-dependent exocytosis of inhibitory neurotransmitters at their contacts with photoreceptor terminals, suggesting that they are capable of releasing GABA via vesicular release. To test whether horizontal cell vesicular release is involved in feedback signaling, we perturbed inhibitory neurotransmission in these cells by targeted deletion of the vesicular GABA transporter (VGAT), the protein responsible for the uptake of inhibitory transmitter by synaptic vesicles. To manipulate horizontal cells selectively, an iCre mouse line with Cre recombinase expression controlled by connexin57 (Cx57) regulatory elements was generated. In Cx57-iCre mouse retina, only horizontal cells expressed Cre protein, and its expression occurred in all retinal regions. After crossing with a VGAT(flox/flox) mouse line, VGAT was selectively eliminated from horizontal cells, which was confirmed immunohistochemically. Voltage-gated ion channel currents in horizontal cells of Cx57-VGAT(-/-) mice were the same as Cx57-VGAT(+/+) controls, as were the cell responses to the ionotropic glutamate receptor agonist kainate, but the response to the GABAA receptor agonist muscimol in Cx57-VGAT(-/-) mice was larger. In contrast, the feedback inhibition of photoreceptor calcium channels, which in control animals is induced by horizontal cell depolarization, was completely absent in Cx57-VGAT(-/-) mice. The results suggest that vesicular release of GABA from horizontal cells is required for feedback inhibition of photoreceptors.

Indexed as

alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic AcidAnimalsCalcium ChannelsConnexinsExcitatory Amino Acid AgonistsFeedback, PhysiologicalFemaleGABA-A Receptor AgonistsGTP-Binding Protein alpha Subunit, Gi2Kainic AcidMaleMembrane PotentialsMiceMice, TransgenicMuscimolPhotoreceptor Cellsalpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic AcidCalcium ChannelsConnexinsExcitatory Amino Acid AgonistsGABA-A Receptor AgonistsGja10 protein, mouseGnai2 protein, mouseGTP-Binding Protein alpha Subunit, Gi2Kainic AcidMuscimolVesicular Inhibitory Amino Acid Transport ProteinsViaat protein, mouseCa channelsCx57-iCreGABA receptorsinhibitory feedbackretinal horizontal cellssynaptic vesicles

Identifiers

PMID27022629
PMCPMC4785380
OpenAlexW2291506557

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.