Evidence map›Paper›PMID 27022622›Full record

ArticleeNeuro

Krüppel-Like Factor 4 (KLF4) Is Not Required for Retinal Cell Differentiation.

Jiahua Fang, Peter X Shaw, Yan Wang, Jeffrey L Goldberg

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eNeuro. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Genetic control of retinal ganglion cell genesis.Cellular and molecular life sciences : CMLS · 2021
    Review
  3. Review
  4. On the Generation and Regeneration of Retinal Ganglion Cells.Frontiers in cell and developmental biology · 2020
    Article
  5. Article
  6. The Role of KLFFrontiers in cellular neuroscience · 2018
    Review
  7. Article
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 2 countries.

Jiahua FangDepartment of Ophthalmology and Shiley Eye Institute, University of California San Diego, La Jolla, California 92093; Department of Ophthalmology, Changsha First People's Hospital, Changsha 410008, Hunan, China.
Peter X ShawDepartment of Ophthalmology and Shiley Eye Institute, University of California San Diego , La Jolla, California 92093.ORCID 0000-0001-7392-6224
Yan WangDepartment of Ophthalmology and Shiley Eye Institute, University of California San Diego , La Jolla, California 92093.
Jeffrey L GoldbergDepartment of Ophthalmology and Shiley Eye Institute, University of California San Diego, La Jolla, California 92093; Byers Eye Institute at Stanford, Palo Alto, California 94303.ORCID 0000-0002-1390-7360
University of California San Diego · US

Funding

Vision BiostatisticsP30EY022589 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Derek Stuart Welsbie · 2012 to 2026
$10.3M
Kruppel-like Transcription Factors in Retinal Ganglion Cell RegenerationR01EY020913 · NEI · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI GOLDBERG, JEFFREY L · 2010 to 2013
$2.2M
HTRA1 as a Therapeutic Target in the Treatment of Wet AMDR01EY025693 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SHAW, PETER X · 2015 to 2019
$1.8M
NEI NIH HHS P30 EY022589NEI NIH HHS P30-EY-022589NEI NIH HHS R01-EY-020913NEI NIH HHS R01 EY025693NEI NIH HHS R01-EY-025693
6 · The paper itself

Abstract

During early vertebrate eye development, a regulatory network of transcription factors regulates retinal cell differentiation and survival into adulthood. Among those factors, Krüppel-like factor 4 (KLF4) plays the dual role of maintaining the stem cell status of retinal progenitors cells and repressing the intrinsic axon regeneration ability in retinal ganglion cells (RGCs) after injury. This study further investigated whether KLF4 plays a role in early retinal cell differentiation or survival into adulthood. We examined different types of retinal neurons, including RGCs, amacrine cells, bipolar cells, Müller cells, and photoreceptor cells, in adult mice in which KLF4 was conditionally deleted in early retinal development using Chx10-promoted Cre by immunohistochemistry. We compared the numbers of retinal neurons and the thickness of photoreceptor and nerve fiber layers between Chx10-Cre-driven KLF4 deletion mice and wild-type mice. There was no significant difference in cell number among any of the retinal cell types or in photoreceptor layer thickness with KLF4 deletion during early development. The thickness of axon bundles in the nerve fiber layer in the Chx10 conditional KLF4 knock-out mice was greater than that in wild-type mice. These results suggest that KLF4 is not required for retinal cell differentiation or survival, but does normally limit retinal ganglion cell axon bundle thickness. These data support a hypothesis that KLF4 suppresses axon growth during development.

Indexed as

Amacrine CellsAnimalsAxonsCell DifferentiationCell SurvivalEpendymoglial CellsFemaleKruppel-Like Factor 4Kruppel-Like Transcription FactorsMaleMiceMice, KnockoutPhotoreceptor Cells, VertebrateRetinaRetinal Bipolar CellsRetinal Ganglion CellsKlf4 protein, mouseKruppel-Like Factor 4Kruppel-Like Transcription Factorsaxon growthKrüppel-like factor 4retinal developmentretinal ganglion cells

Identifiers

PMID27022622
PMCPMC4770008
OpenAlexW2288562491

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.