ArticlePharmacology research & perspectives2015
Activation of transient receptor potential ankyrin 1 induces CGRP release from spinal cord synaptosomes.
Article in Pharmacology research & perspectives, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
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- Roles of calcitonin gene-related peptide in the skin, and other physiological and pathophysiological functions.Brain, behavior, & immunity - health · 2021Review
- Painful diabetic neuropathy leads to functional CaExperimental neurology · 2021Article
- Identification of herbal components as TRPA1 agonists and TRPM8 antagonists.Journal of natural medicines · 2021Article
- Differential sensitivity of cinnamaldehyde-evoked calcium fluxes to ruthenium red in guinea pig and mouse trigeminal sensory neurons.BMC research notes · 2021Article
- Reply to "TRPA1-dependent calcium transients and CGRP release in DRG neurons require extracellular calcium".The Journal of cell biology · 2020Article
- TRPA1-dependent calcium transients and CGRP release in DRG neurons require extracellular calcium.The Journal of cell biology · 2020Article
- A Review of the Effects of Pain and Analgesia on Immune System Function and Inflammation: Relevance for Preclinical Studies.Comparative medicine · 2019Review
- Sensory nerves mediate spontaneous behaviors in addition to inflammation in a murine model of psoriasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Protective effects of voltage-gated calcium channel antagonists against zinc toxicity in SN56 neuroblastoma cholinergic cells.PloS one · 2018Article
- The Role of Calcitonin Gene Related Peptide (CGRP) in Neurogenic Vasodilation and Its Cardioprotective Effects.Frontiers in physiology · 2018Review
- Local NGF and GDNF levels modulate morphology and function of porcine DRG neurites, In Vitro.PloS one · 2018Article
- Activation of transient receptor potential ankyrin 1 induces CGRP release from spinal cord synaptosomes.Pharmacology research & perspectives · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transient receptor potential ankyrin 1 (TRPA1) is a sensor of nociceptive stimuli, expressed predominantly in a subpopulation of peptidergic sensory neurons which co-express the noxious heat-sensor transient receptor potential vanilloid 1. In this study, we describe a spinal cord synaptosome-calcitonin gene-related peptide (CGRP) release assay for examining activation of TRPA1 natively expressed on the central terminals of dorsal root ganglion neurons. We have shown for the first time that activation of TRPA1 channels expressed on spinal cord synaptosomes by a selection of agonists evokes a concentration-dependent release of CGRP which is inhibited by TRPA1 antagonists. In addition, our results demonstrate that depolarization of spinal cord synaptosomes by a high concentration of KCl induces CGRP release via a T-type calcium channel-dependent mechanism whilst TRPA1-induced CGRP release functions independently of voltage-gated calcium channel activation. Finally, we have shown that pre-treatment of synaptosomes with the opioid agonist, morphine, results in a reduction of depolarization-induced CGRP release. This study has demonstrated the use of a dorsal spinal cord homogenate assay for investigation of natively expressed TRPA1 channels and for modulation of depolarizing stimuli at the level of the dorsal spinal cord.
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