ArticleMolecular and biochemical parasitology
Real-time PCR for sexing Schistosoma mansoni cercariae.
Article in Molecular and biochemical parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Heritable transgenic schistosomes as a living platform for SARS-CoV-2 neutralizing antibody secretion.Nature communications · 2026Article
- A male-pheromone-elevated transcription factor ZNF362.1 in female schistosomes determines sexual maturation.Science advances · 2026Article
- Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke,Cell reports methods · 2023Article
- No evidence for schistosome parasite fitness trade-offs in the intermediate and definitive host.Parasites & vectors · 2023Article
- Genetic analysis of praziquantel response in schistosome parasites implicates a transient receptor potential channel.Science translational medicine · 2021Article
- Genetic architecture of transmission stage production and virulence in schistosome parasites.Virulence · 2021Article
- Satellite-Like W-Elements: Repetitive, Transcribed, and Putative Mobile Genetic Factors with Potential Roles for Biology and Evolution of Schistosoma mansoni.Genome biology and evolution · 2021Article
- Validation of a human-serum-based in vitro growth method for drug screening on juvenile development stages of Schistosoma mansoni.PLoS neglected tropical diseases · 2021Article
- Duplex real-time PCR for sexing Schistosoma japonicum cercariae based on W chromosome-specific genes and its applications.PLoS neglected tropical diseases · 2020Article
- Striking differences in virulence, transmission and sporocyst growth dynamics between two schistosome populations.Parasites & vectors · 2019Article
- Multiplex PCR for sexing Schistosoma japonicum cercariae and its utility.Parasitology research · 2019Article
- Genetic Crosses and Linkage Mapping in Schistosome Parasites.Trends in parasitology · 2018Review
- A Genome Wide Comparison to Identify Markers to Differentiate the Sex of Larval Stages of Schistosoma haematobium, Schistosoma bovis and their Respective Hybrids.PLoS neglected tropical diseases · 2016Article
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Authors and funding
5 authors.
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Abstract
The gender of cercarial larvae can only be determined using molecular methods. End point PCR methods that amplify repetitive markers on the W chromosome of the female (ZW) parasites have been developed, but sometimes results are ambiguous or incorrect. To more effectively distinguish sexes, and to determine why end point PCR can be incorrect, we quantified the W6 repeat sequence and a specific Z chromosome gene using real-time PCR. The ratio between copy number of W6 and a Z chromosome marker unambiguously identifies gender: females have higher ratios (421-4371) than males (0-21). However, some males have low numbers of W6 elements in their genome, and qPCR demonstrated significantly higher W6/Z marker ratios for male genotypes giving ambiguous end point PCR results compared with males giving clear end point results. The quantitative PCR sexing method developed will be particularly useful where reliable sexing of cercariae is critical, for example when staging genetic crosses.
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