ArticleInfection and immunity2016
Determination of Active Phagocytosis of Unopsonized Porphyromonas gingivalis by Macrophages and Neutrophils Using the pH-Sensitive Fluorescent Dye pHrodo.
Article in Infection and immunity, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Macrophages transfer functional proteins to their surface during live-cell uptake.Nature chemical biology · 2026Article
- A Ratiometric pH Sensor for Gram-Positive and Gram-Negative Bacteria.Journal of the American Chemical Society · 2026Article
- Macrophage activation and invasion byJournal of oral microbiology · 2026Article
- c-Myc Inhibits Macrophage Antimycobacterial Response in Mycobacterium tuberculosis Infection.The Journal of infectious diseases · 2025Article
- The development of a direct co-culture-based model for diabetic foot ulcer mimicking inflammation and impaired phagocytosis.In vitro models · 2025Article
- c-Myc inhibits macrophage antimycobacterial response inbioRxiv : the preprint server for biology · 2025Article
- Macrophage-Hosted Porphyromonas gingivalis Is a Risk Factor for Cataract Development.Investigative ophthalmology & visual science · 2025Article
- Dietary fatty acids differentially impact phagocytosis, inflammatory gene expression, and mitochondrial respiration in microglial and neuronal cell models.Frontiers in cellular neuroscience · 2023Article
- Differential regulation of Type 1 and Type 2 mouse eosinophil activation by apoptotic cells.Frontiers in immunology · 2022Article
- Real-time visualization of phagosomal pH manipulation byFrontiers in cellular and infection microbiology · 2022Article
- Phagocytic activity of monocytes and neutrophils in patients with periodontitis, whether or not associated to type 2 diabetes.Acta odontologica latinoamericana : AOL · 2021Article
- tiRNA signaling via stress-regulated vesicle transfer in the hematopoietic niche.Cell stem cell · 2021Article
- Periodontal Pathogens' strategies disarm neutrophils to promote dysregulated inflammation.Molecular oral microbiology · 2021Review
- Article
- Imaging of the immune system - towards a subcellular and molecular understanding.Journal of cell science · 2020Review
- A Common Genetic Variation in Langerin (CD207) Compromises Cellular Uptake of Staphylococcus aureus.Journal of innate immunity · 2020Article
- Inhibition of osteoclastogenesis by opsonizedFASEB bioAdvances · 2019Article
- Unprimed, M1 and M2 Macrophages Differentially Interact with Porphyromonas gingivalis.PloS one · 2016Article
Corrections and comments
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Phagocytosis of pathogens is an important component of the innate immune system that is responsible for the removal and degradation of bacteria as well as their presentation via the major histocompatibility complexes to the adaptive immune system. The periodontal pathogen Porphyromonas gingivalis exhibits strain heterogeneity, which may affect a phagocyte's ability to recognize and phagocytose the bacterium. In addition, P. gingivalis is reported to avoid phagocytosis by antibody and complement degradation and by invading phagocytic cells. Previous studies examining phagocytosis have been confounded by both the techniques employed and the potential of the bacteria to invade the cells. In this study, we used a novel, pH-sensitive dye, pHrodo, to label live P. gingivalis strains and examine unopsonized phagocytosis by murine macrophages and neutrophils and human monocytic cells. All host cells examined were able to recognize and phagocytose unopsonized P. gingivalis strains. Macrophages had a preference to phagocytose P. gingivalis strain ATCC 33277 over other strains and clinical isolates in the study, whereas neutrophils favored P. gingivalis W50, ATCC 33277, and one clinical isolate over the other strains. This study revealed that all P. gingivalis strains were capable of being phagocytosed without prior opsonization with antibody or complement.
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