ArticleInternational orthopaedics2016
Hamstring graft bacterial contamination during anterior cruciate ligament reconstruction: clinical and microbiological study.
Article in International orthopaedics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Vancomycin-soaking of the graft reduces the incidence of septic arthritis following ACL reconstruction: results of a systematic review and meta-analysis.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2020Pooled it
- Evaluation the results of anterior cruciate ligament reconstruction with or without using antibiotic solution during graft preparation.European journal of orthopaedic surgery & traumatology : orthopedie traumatologie · 2023Trial
- Article
- Bacterial Analysis of Hamstring Autograft With Vancomycin Presoaking in Primary Anterior Cruciate Ligament Reconstruction Shows High Incidence of Colonization Without Early Clinical Infection.Arthroscopy, sports medicine, and rehabilitation · 2025Article
- Can contaminated musculoskeletal autografts and allografts Be safely and effectively used in Patients? A systematic review.Journal of orthopaedics · 2025Review
- Soaking in Povidone-Iodine, Chlorhexidine, Teicoplanin, Vancomycin, and Saline Solution Differentially Alters Porcine Flexor Tendon Size and Biomechanical Properties.Arthroscopy, sports medicine, and rehabilitation · 2025Article
- Effectiveness of a super-oxidized solution for decontaminating ACL grafts: a prospective study.Journal of orthopaedic surgery and research · 2025Article
- A Systematic Review and Meta-analysis of Risk Factors for an Infection After Anterior Cruciate Ligament Reconstruction.Orthopaedic journal of sports medicine · 2023Review
- Allograft contamination during suture preparation for anterior cruciate ligament reconstruction: an ex vivo study.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2022Article
- Evaluating bacterial contamination of free bone fragments falling to the ground and the effectiveness of antiseptic solutions.European journal of orthopaedic surgery & traumatology : orthopedie traumatologie · 2022Article
- Does Vancomycin Wrapping in Anterior Cruciate Ligament Reconstruction Affect Tenocyte Activity In Vitro?Antibiotics (Basel, Switzerland) · 2021Article
- Comparison of the Sterilization Efficiency of 3 Disinfectants for Dropped Anterior Cruciate Ligament Grafts: A Systematic Review and Meta-analysis.Orthopaedic journal of sports medicine · 2021Review
- Intraoperative graft decontamination during ACL reconstruction surgery.Revista brasileira de ortopedia · 2020Article
- Vancomycin pre-soaking of the graft reduces postoperative infection rate without increasing risk of graft failure and arthrofibrosis in ACL reconstruction.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2019Article
- Hamstring Tendon Autograft Contamination in Anterior Cruciate Ligament Reconstruction: Comparison between two Harvesting Techniques.Revista brasileira de ortopedia · 2019Article
- Orthopaedic publications from Egypt in the last five years: a bibliometric report.International orthopaedics · 2018Review
- Hamstring autografts are associated with a high rate of contamination in anterior cruciate ligament reconstruction.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2018Article
- Contamination occurs during ACL graft harvesting and manipulation, but it can be easily eradicated.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2018Article
- Does a different dose of gamma irradiation have the same effect on five different types of tendon allografts? - a biomechanical study.International orthopaedics · 2017Article
- Delayed Tibial Osteomyelitis after Anterior Cruciate Ligament Reconstruction with Hamstrings Autograft and Bioabsorbable Interference Screw: A Case Report and Review of the Literature.Case reports in orthopedics · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeAutograft preparation for anterior cruciate ligament (ACL) reconstruction has a potential for graft contamination. The purpose of this study was to evaluate the possibility of bacterial contamination of hamstring autograft during preparation and when dropped onto the operating room floor and methods of graft decontamination.
methodsSixty hamstring tendon autograft specimens were used as the test group. Excess tendon not used in the ACL procedure was divided into five segments. One segment, at the completion of preparation, was sent for culture as a control; the remaining four segments were dropped onto the floor adjacent to the surgical field for 15 seconds. One segment was cultured without undergoing any further treatment. Cultures were taken from each segment after immersion in 10 % povidone-iodine solution, 4 % chlorhexidine and bacitracin, respectively, for three minutes. Cultures of a skin swab and floor swab were taken at the same time and place that the ACL was dropped.
resultsCultures of control graft tissue from ten patients (16.7 %) were positive for bacteria. No patient developed post-operative infection. Ninety organisms were identified, with Staphylococcus epidermidis being the most common isolate. Grafts rinsed in either bacitracin or 4 % chlorhexidine solutions were less likely to be culture positive.
conclusionsA high rate of contamination can be expected during autograft preparation for ACL reconstruction. Soaking the hamstring autograft in either bacitracin or 4 % chlorhexidine solution is effective for decontamination, particulary if graft is dropped on the floor.
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